四妙勇安汤不同配伍组合中环烯醚萜苷类成分肠吸收研究

Intestinal absorption of iridoid glycosides in Simiao Yong’an Tang with different compatibility

目的考察四妙勇安汤水提物中环烯醚萜苷类成分在不同肠段中、不同配伍组合时的肠吸收情况。方法建立大鼠在体单向肠灌流模型,采用UPLC-MS^n法测定不同肠段灌流液中哈巴苷、当药苷、马钱苷和哈巴俄苷4种环烯醚萜苷类成分的含量,计算其在不同肠段中的吸收速率常数(Ka)和表观渗透系数(Papp),并以此为指标评价环烯醚萜苷类成分肠吸收情况。结果四妙勇安汤不同配伍组合中环烯醚萜苷类成分在回肠和十二指肠中的吸收情况较空肠和结肠好(P<0.05)。回肠中4种环烯醚萜苷类成分的肠吸收参数均在金银花-玄参-当归3药配伍时最高。十二指肠中马钱苷、当药苷在金银花-当归配伍时表现出较高的肠吸收参数;而哈巴苷、哈巴俄苷在复方中肠吸收参数最高,单味药中最低。结论环烯醚萜苷类成分在配伍组合时的肠吸收参数(Ka、Papp值)较所含该类成分的单味药材高。在一定程度上表明复方配伍可促进环烯醚萜苷类成分的吸收,改善其口服生物利用度低的问题。

Objective To investigate the intestinal absorption of iridoid glycosides from Simiao Yong'an Tang aqueous extract in different combination. Methods The rat single pass intestinal perfusion model was established. The contents of harpagide, sweroside, loganin and harpagoside from various intestinal segment perfusates were determined by using UPLC-MS^n. As an evaluation index for intestinal absorption, the absorption rate constant(Ka) and the apparent permeability coefficient(Papp) of iridoid glycosides from Simiao Yong'an Tang in different intestinal section were calculated. Results In the ileum and duodenum, the absorption of iridoid glycosides from Simiao Yong'an Tang with different combination was better than that in jejunum and colon(P<0.05). The intestinal absorption parameters of four iridoid glycosides in ileum were the highest in the compatibility of jinyinhua(Lonicerae Japonicae Flos), xuanshen(Scrophulariae Radix) and danggui(Angelicae Sinensis Radix). In duodenum, sweroside, loganin showed higher intestinal absorption parameters when gancao(Glycyrrhizae Radix et Rhizoma) was compatible with danggui, while the intestinal absorption parameters of harpagide and harpagoside were the highest in the prescription and the lowest in the single herb. Conclusions The intestinal absorption parameters of iridoid glycosides were higher in prescription than those in single herb. To some extent, these results indicated that the compatibility could promote the absorption of iridoid glycosides and improve the problem of low oral bioavailability.