桃细菌性穿孔病菌遗传多样性研究

Genetic diversity of Xanthomonas arboricola pv. pruni strains in China

为了解我国桃细菌性穿孔病菌(Xanthomonas arboricola pv.pruni)菌株间的分子水平差异,以来自我国桃主产区的51株桃细菌性穿孔病菌株为研究对象,采用rep-PCR和ISSR-PCR方法进行UPGMA聚类分析。结果表明:使用REP和ERIC引物能够得到较好的遗传多样性分析结果,均拥有9个指纹位点,且分别有17和26种指纹谱型,当遗传相似水平为0.55和0.80时可将所有菌株分别划分为6和10簇组群。聚类分析表明我国桃细菌性穿孔病菌菌株间的同源性较高,但也具有一定的多样性,这与菌株来自不同地理产区存在一定的相关性。这一研究为不同地区桃细菌性穿孔病菌监测与病害防控提供了科学依据。

Bacterial shot hole is an important disease of peach and is caused by Xanthomonas arboricola pv. pruni(Xap). In order to understand the genetic diversities of Xap in China, 51 Xap strains collected from peach orchards representing four provinces in China were fingerprinted by repetitive sequence-based(rep)-PCR and inter-simple sequence repeat(ISSR)-PCR. Dendrograms were generated by NTSYS software according to fingerprint features. The results indicated that 51 strains had better genetic diversities using REP and ERIC primers, and both had 9 fingerprint sites and 17 and 26 fingerprint profiles respectively. All strains could be divided into 6 and 10 clusters at genetic similarity levels of 0.55(REP) and 0.80(ERIC), respectively. Cluster analysis showed that there was high homology among the Xap strains, but there was also some diversity, and this diversity had a certain level of correlation with different geographical locations. The result from the present study may provide a scientific basis for monitoring the pathogen and controlling peach bacterial shot hole in different peach-growing areas.