杨桃根多糖提取工艺优化及其体外活性

Extraction process optimization and in vitro activity evaluation for polysaccharides from Averrhoa carambola roots

目的优化杨桃Averrhoa carambola L.根多糖提取工艺,并评价其体外活性。方法在单因素试验基础上,以提取温度、提取时间、料液比为影响因素,多糖提取率为评价指标,正交试验优化提取工艺。然后,酶标板法考察多糖抗氧化活性及其对α-葡萄糖苷酶的抑制作用。结果最佳条件为提取温度70℃,提取时间30 min,料液比1∶30,多糖提取率1.93%。多糖对ABTS^·+、DPPH·的EC50值分别为0.10、0.33 mg/mL,对α-葡萄糖苷酶的抑制率高于阿卡波糖。结论该方法稳定可靠,可用于提取具有较强抗氧化活性、抑制α-葡萄糖苷酶作用的杨桃根多糖。

AIM To optimize the extraction process for polysaccharides from Averrhoa carambola L. roots and to evaluate their in vitro activities.METHODS With extraction temperature, extraction time and solid-liquid ratio as influencing factors, the extraction rate of polysaccharides as an evaluation index, the extraction process was optimized by orthogonal test on the basis of single factor test. Subsequently, enzyme label plate assay was applied to investigating polysaccharides’ antioxidant activity and inhibitory effect on α-glucosidase.RESULTS The optimal conditions were determined to be 70 ℃ for extraction temperature, 30 min for extraction time, and 1∶30 for solid-liquid ratio, the extraction rate of polysaccharides was 1.93%. The EC50 values of polysaccharides on ABTS^·+, DPPH·were 0.10, 0.33 mg/mL, respectively, whose inhibitory rate on α-glucosidase was higher than that of acarbose.CONCLUSION This stable and reliable method can be used for extracting polysaccharides, with strong antioxidant activity and inhibitory effect on α-glucosidase from A. carambola roots.