LncRNA MALAT1在结肠癌组织中的表达及其与预后的相关性研究

The Expression of Long Non-coding RNA MALAT1 in Colon Cancer and Its Correlation with Prognosis

目的 探讨长链非编码RNA(long non-coding RNA, LncRNA)肺癌转移相关转录本1(metastasis-associated lung adenocarcinoma transcript 1, MALAT1)在结肠癌组织中的表达及其与预后的相关性。方法 选择我院收治的89例结肠癌,均行根治性切除手术,术中取肿瘤组织及癌旁正常组织。采用RT-PCR法检测LncRNA MALAT1表达情况,记录所有患者的临床资料,观察总生存期(overall survival, OS)、无进展生存期(progression free survival, PFS)及术后3年复发和转移情况,比较LncRNA MALAT1在不同临床特征的表达情况及其与预后的相关性。结果 结肠癌组织、癌旁正常组织LncRNA MALAT1相对表达水平分别为2.84±0.41、0.89±0.36,比较差异有统计学意义( t =2.977、 P <0.05)。LncRNA MALAT1高表达组与低表达组的TNM分期、分化程度、浸润深度、血管浸润、淋巴结转移比较差异具有统计学意义( P <0.05)。本组24例出现局部复发,复发率为26.97%;43例出现远处转移,发生率为48.31%。LncRNA MALAT1高表达组局部复发及远处转移发生率均显著高于低表达组,比较差异有统计学意义( P <0.05)。Kaplan-Meier法分析显示, LncRNA MALAT1高表达组中位OS、中位PFS均低于低表达组,比较差异有统计学意义(χ^2=4.146、 P <0.05,χ^ 2=5.205、 P <0.05)。多因素Cox回归分析显示,TNM分期Ⅲ期+Ⅳ期、分化程度为中分化+高分化、浸润深度侵及浆膜层、有淋巴结转移、LncRNA MALAT1高表达是影响结肠癌患者预后的独立危险因素( P <0.05)。结论 结肠癌组织高表达LncRNA MALAT1,且高水平LncRNA MALAT1与结肠癌患者不良预后密切相关。

Objective To investigate the expression of long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in colon cancer and its correlation with prognosis. Methods Eighty-nine patients with colon cancer were selected for radical resection and their tumor tissues and adjacent normal tissues were collected intraoperatively. The expression of LncRNA MALAT1 was detected by RT-PCR, and the patient's clinical data were recorded. Overall survival (OS), progression free survival (PFS), and 3-year recurrence and metastasis postoperatively were observed. The expression of LncRNA MALAT1 in patients with different clinical features and its correlation with prognosis were compared. Results The relative expression levels of LncRNA MALAT1 in colon cancer tissues and adjacent normal tissues were 2.84±0.41 and 0.89±0.36, respectively, and there were significant differences ( t =2.977, P <0.05). There was a significant difference in different TNM stages, degrees of differentiation, depth of infiltration, vascular infiltration and lymph node metastasis between LncRNA MALAT1 high expression group and low expression group ( P <0.05). Local recurrence occurred in 24 patients in this group, and the recurrence rate was 26.97%;43 patients had distant metastasis, and the incidence rate was 48.31%. The incidence of local recurrence and distant metastasis in the LncRNA MALAT1 high expression group was significantly higher than that in the low expression group ( P <0.05). Kaplan-Meier analysis showed that the median OS and PFS of the LncRNA MALAT1 high expression group were significantly lower than those of the low expression group (χ 2=4.146, P < 0.05;χ 2=5.205, P <0.05). Multivariate Cox regression analysis showed that TNM stage III+stage IV, moderate differentiation+ high differentiation, deep infiltration to serosal layer, lymph node metastasis, and high expression of LncRNA MALAT1 were independent risk factors for prognosis of colon cancer patients ( P <0.05). Conclusion LncRNA MALAT1 is highly expressed in colon cancer tissues, which is closely related to poor prognosis in colon cancer patients.