盐生肉苁蓉通便有效部位的提取工艺及药效学研究

Study on extraction technology and pharmacodynamics of laxation effective parts of Cistanche salsa

目的研究盐生肉苁蓉通便活性部位的最佳提取工艺及药效学.方法以半乳糖醇含量和浸膏得率为考察指标,采用单因素考察和正交实验法优化提取工艺.采用地芬诺酯建立小鼠便秘模型,通过小鼠小肠运动及小鼠排便实验考察盐生肉苁蓉提取物的通便作用.结果盐生肉苁蓉通便部位的最佳提取工艺条件:乙醇浓度为40%,料液比为1∶16(g/mL),超声提取120 min,提取3次,该提取物中半乳糖醇含量为20.1%.盐生肉苁蓉通便部位能明显缩短便秘模型小鼠首次排便时间(P<0.01),增加6h内粪便粒数、粪便质量和炭末推进率(P<0.01).结论盐生肉苁蓉通便有效部位提取工艺合理、经济简便,可为肉苁蓉润肠通便保健品开发提供技术参数.

Objective To investigate optimum extraction process and pharmacodynamics of laxation effective parts of Cistanche salsa.Methods Content of dulcitol and the extract yield as parameters,single factor screening and orthogonal experiments were used to optimize the optimum extraction process.Constipation mice model was established by oral administration with diphenoxylate,the intestine motor and laxation experiments were used to investigate laxation pharmacodynamics of the extract.Results The optimum extraction process of C.salsa laxative extract were as follows:ethanol concentration was 40%,material liquid ratio was 1:16(g/mL),ultrasonic extraction time was 120 min for 3 times.The content of dulcitol in laxative extract was 20.1%.Compared to model group,different dosage of extracts could obviously shorten the first defecation time(P<0.01),and increase dejection amounts,feces weights and charcoal propulsion rate in 6 hours(P<0.01).Conclusion This extraction process of C.salsa laxative extract is reasonable,economic and easy,which can provide technical parameters for the development of C.salsa.