芜菁酸性多糖BRAP-2体内抗Lewis肺癌活性研究

Study on the anti-Lewis lung cancer activity of Brassica rapa L. acid polysaccharide-2 in vivo

目的评价芜菁多糖的急性毒性并研究芜菁酸性多糖BRAP-2(Brassica rapa L.acide polysaccharide-2)体内抗Lewis肺癌(Lewis lung cancer,LLC)活性.方法通过急行毒性实验、半数致死量(median lethal dose)测定和最大耐受量(maximal tolerable dose,MTD)实验来评价芜菁多糖的急性毒性;采用CCK-8法检测Lewis肺癌细胞的生长曲线;通过计算Lewis肺癌小鼠肿瘤体积、抑瘤率及有效体质量来研究芜菁酸性多糖BRAP-2抗Lewis肺癌活性.结果芜菁多糖的MTD为28 g/kg·d,毒性作用低;LLC细胞在培养的前4d进入对数生长期,结果具有统计学意义(P<0.01);与模型组比,芜菁酸性多糖BRAP-2各剂量组和DDP组的瘤重均显著降低,抑瘤率明显升高,差异均有统计学意义(P<0.05),芜菁酸性多糖BRAP-2高、中、低剂量组和DDP组抑瘤率分别为51.45%、46.57%、19.53%和76.83%,造模前和造模给药后各实验小鼠体质质量发生不同程度的变化,差异均有统计学意义(P<0.05).结论芜菁多糖毒性作用低;Lewis肺癌细胞培养的第4d可作为传代、冻存和制造LLC肺癌模型的最佳时间段;可推测芜菁酸性多糖BRAP-2体内具有较好的抑瘤活性,同时多糖类成分因具有提高机体免疫力和抗肿瘤,低毒、高效、安全、副作用小等特点临床上可作为优选的抗肿瘤药.

Objective To evaluate the acute toxicity of Brassica rapa L.polysaccharide and investigate the anti-Lewis lung cancer activity of Brassica rapa L.acid polysaccharide(BRAP-2)in vivo.Methods The acute toxicity of Brassica rapa L.polysaccharide was evaluated by acute toxicity test,median lethal dose test and maximal tolerable dose(MTD)test.The growth curve of Lewis lung cancer cells was measured by CCK-8 method.Anti-Lewis lung cancer activity of Brassica rapa L.acid polysaccharide BRAP-2 was studied by calculating tumor volume,inhibition rate and effective body mass of Lewis lung cancer mice.Results The MTD of Brassica rapa L.polysaccharide was 28 g/kg·d,which indicated that the toxicity of Brassica rapa L.polysaccharide was low.LLC cells entered logarithmic growth stage in the first 4 days of culture(P<0.01).Compared with model group,each dose group and DDP group of Brassica rapa L.acid polysaccharide BRAP-2 had significant antitumor activity.The tumour weights significantly decreased and the tumour inhibition rates increased significantly(P<0.05).The tumour inhibition rates of high,medium and low dose of BRAP-2 group and DDP group were 51.45%,46.57%,19.53%and 76.83%,respectively.There were significant differences in the body quality of each experimental mice before and after the administration of BRAP-2(P<0.05).Conclusion Brassica rapa L.polysaccharide has low toxicity.The first 4 days of Lewis lung cancer cell culture can be used as the best time for passage,cryopreservation and modeling of LLC lung cancer model.Experiments preliminarily speculate that Brassica rapa L.acid polysaccharide has good anti-tumor activity in vivo,and meanwhile has the characteristics of low toxicity,high efficiency,safety,little side effects,improving immunity and anti-tumor,etc,which can be used as an antineoplastic drug in clinic.