复方万年青胶囊对大鼠血管性痴呆的改善作用

Improvement of Compound Wannianqing Capsules on vascular dementia of rats

目的探讨复方万年青胶囊对大鼠血管性痴呆的改善作用及机制。方法采用双侧颈总动脉永久性结扎法建立大鼠血管性痴呆模型,将40只SD大鼠随机分成假手术组、模型组、复方万年青胶囊0.375 g/kg组和尼麦角林0.011 g/kg组,每组10只,连续ig给药45 d,每天1次。采用Morris水迷宫实验考察大鼠寻找站台潜伏期、原象限停留时间比和距离比,并检测生化指标IL-6、乙酰胆碱酯酶(AchE)的水平。结果与模型组相比,复方万年青胶囊组大鼠在第1、2、4、5天寻找站台的潜伏期均明显缩短(P<0.05),在原象限停留时间比和距离比均明显增加(P<0.05),生化指标AchE水平明显降低(P<0.01),细胞因子IL-6水平降低,无显著性差异。结论复方万年青胶囊具有改善大鼠血管性痴呆的作用,可能与降低AchE活性,提升乙酰胆碱水平,修复受损的中枢胆碱能神经系统有关。

Objective To explore the improvement of Compound Wannianqing Capsules on vascular dementia of rats and its mechanism. Methods Vascular dementia rats model was established by bilateral carotid artery ligation method. SD rats(40) were randomly divided into sham group, model group, Compound Wannianqing Capsules 0.375 g/kg group, and nicergoline 0.011 g/kg group, and each group had 10 rats. Rats were ig administered with drugs for 45 d, once daily.The incubation period of rats finding the latency of the platform, the time ratio of the original quadrant, and the distance ratio were observed by the Morris water maze. The biochemical index IL-6 and acetylcholinesterase(AchE) levels were detected. Results Compared with the model group, the incubation period of Compound Wannianqing Capsules group was significantly shortened in 1, 2, 4, and 5 d(P < 0.05), and the original quadrant and the distance ratio were significantly increased(P < 0.05), and the biochemical indicators of AchE was significantly decreased(P < 0.01), and the level of cytokine IL-6 was reduced with no significant difference. Conclusion Compound Wannianqing Capsule has the improvement effect of vascular dementia in rats, and the mechanism may be related to reducing AchE activity, increasing acetylcholine levels, and repairing damaged central cholinergic nervous system.