丝素/壳聚糖/沙蒿籽胶复合水凝胶的制备与表征

Preparation and characterization of silk fibroin/chitosan/artemisia argyi(SF/CS/SA)composite hydrogel

目的:制备丝素/壳聚糖/沙蒿籽胶(SF/CS/SA)复合水凝胶,并进行性能表征和进一步优化,为制备性能优异的神经移植物提供依据。方法:采用溶胶-凝胶方法制备SF/CS/SA复合水凝胶,根据成胶效果进行初步筛选,然后对筛选出的样品进行成胶时间、孔隙率、溶胀率、降解残留率和杨氏模量分析。结果:(1)通过丝素、壳聚糖和沙蒿籽胶的不同比例可以制备出SF/CS/SA复合水凝胶,其中9组成胶完整且稳定,编号A-I,进行后续实验。(2)A、B、C、G、H、I组成胶时间较快,且成胶效果好;复合水凝胶的孔隙率主要集中60%和80%附近,溶胀率变化与孔隙率呈正相关;A、B、G、H、I组降解性能良好;力学性能测定发现A、B组柔性较好,而C、G、H组脆性较好。结论:筛选出成胶时间较快、溶胀性能较好、降解速度较慢、力学性能优良的A、B、G、H组丝素/壳聚糖/沙蒿籽胶复合水凝胶,为制备性能优异的神经移植物提供重要参考。

Objective: Preparing silk fibroin/chitosan/artemisia argyi composite hydrogel and characterizing the properties of the hydrogel, in order to obtain the optimized composite hydrogel for providing reference of developing nerve implants. Methods: Firstly, the SF/CS/SAcomposite hydrogel was prepared by using sol-gel method, the preliminary screening was carried out to evaluate the gelation effect. The gelation time, porosity, swelling ratio, degradation ratio and Young’s modulus of the selected samples were analyzed and further optimized. Results:(1) The macroscopic observation of the gelation effect of the composite hydrogel showed that the SF/CS/SA composite hydrogel could be prepared by adjusting different ratios of silk fibroin, chitosan and artemisia argyi gum, among which 9 groups were complete and stable, and were numbered as A-I.(2) The gelation time showed that the composition of A, B, C, G, H and I was faster and the gelation effect was better. The porosity test results showed that the porosity of the composite hydrogel was mainly concentrated 60% and 80. The swelling ratio showed that the change of swelling ratio was positively correlated with the porosity;the degradation performance of the composite hydrogel displayed that the degradation performance of Group A, B, G, H and I was good. The mechanical properties demonstrated that the A and B groups had better flexibility, while the C,G and H groups had better brittleness. Conclusion: The A, B, G and H groups with faster gelation time, better swelling performance, slower degradation rate and excellent mechanical properties may have potential application for the development of tissue engineered nerve grafts.