麻疹病毒检测中实时荧光定量RT-PCR的价值分析

Value Analysis of Real-Time Fluorescence Quantitative RT-PCR in Measles Virus Detection

目的评价麻疹病毒检测中实时荧光定量RT-PCR的应用价值。方法选取疾病预防控制中心在2013年4月至2014年12月期间收治的70例疑似麻疹病例,采集患者血清标本及咽拭子标本,分别采取酶联免疫吸附试验(ELISA)法、RT-PCR法检测;比较两种检测技术检测结果。结果 ELISA法检测血清标本IgM抗体阳性率10.77%,RT-PCR检测阳性率为24.62%,数据具显著性差异(χ^2=4.279,P=0.039);ELISA法于出疹1~3d内总检测阳性率为42.86%,RT-PCR法于出疹1~3d内总检测阳性率为93.75%,数据差异具显著性(P<0.05)。结论对麻疹病毒采取实时荧光定量RT-PCR检测,具较高的灵敏度,操作简单,值得临床推广。

objective To evaluate the application value of real -time fluorescence quantitative RT -PCR in measles virus detection. Methods Seventy suspected measles cases admitted to the CDC from April 2013 to December 2014 were selected. Serum samples and swab samples were collected and detected by enzyme-linked immunosorbent assay (ELISA) and RT-PCR,respectively. The results of the two detection techniques were compared. Results The positive rate of IgM antibody was 10.77% by ELISA and 24.62% by RT-PCR. The data showed significant difference (x^2=4.279, P=0.039);the total positive rate of ELISA was 42.86% within 1-3 days of eruption,and the total positive rate of RT-PCR was 93.75% within 1-3 days of eruption,with significant difference (P<0.05). Conclusion Real-time fluorescence quantitative RT-PCR detection of measles virus has high sensitivity and simple operation.