摘要
The heart is regulated by excitation-contraction mechanism and electrical stimulation, so both cardiac tissues and myocytes in vivo are affected by the mechanical and electrical factors at the same time. The device that can supply both tensile stimulation and electrical stimulation was produced. Cardiomyocytes were isolated by trypsin and collagenase Ⅱ combined digestion. After 72 h's culture, different electrical stimulations(0.5 V/cm, 1 V/cm, 2 V/cm;1 Hz;2 ms;30 min) and mechanical stretching force(tensile 10%, 6 h) were used to stimulate cultured cardiomyocytes respectively. Then the combinative stimulation of mechanical and electrical stimulation with 0.5 V/cm was used to apply to cardiomyocytes simultaneously. After stimulation,Connexin-43(Cx-43) mRNA level was detected by RT-PCR. The stimulation by electrical pulse of 0.5 V/cm resulted in obvious enhancement of Cx-43 m RNA level compared to the control group and the Cx-43 mRNA levels at 0.5 V/cm were higher significantly than those under both 1.0 V/cm and 2.0 V/cm. After 10% of tensile stimulation for 6 h, expressions of Cx-43 mRNA were also significantly promoted. The combinative effect of mechanical and electrical stimulation on the expression of Cx-43 mRNA is higher than that under unique tensile stimulation or electrical stimulation,which is more beneficial to the development and connection of cardiomyocytes.
The heart is regulated by excitation-contraction mechanism and electrical stimulation, so both cardiac tissues and myocytes in vivo are affected by the mechanical and electrical factors at the same time. The device that can supply both tensile stimulation and electrical stimulation was produced. Cardiomyocytes were isolated by trypsin and collagenase Ⅱ combined digestion. After 72 h’s culture, different electrical stimulations(0.5 V/cm, 1 V/cm, 2 V/cm; 1 Hz; 2 ms; 30 min) and mechanical stretching force(tensile 10%, 6 h) were used to stimulate cultured cardiomyocytes respectively. Then the combinative stimulation of mechanical and electrical stimulation with 0.5 V/cm was used to apply to cardiomyocytes simultaneously. After stimulation,Connexin-43(Cx-43) mRNA level was detected by RT-PCR. The stimulation by electrical pulse of 0.5 V/cm resulted in obvious enhancement of Cx-43 m RNA level compared to the control group and the Cx-43 mRNA levels at 0.5 V/cm were higher significantly than those under both 1.0 V/cm and 2.0 V/cm. After 10% of tensile stimulation for 6 h, expressions of Cx-43 mRNA were also significantly promoted. The combinative effect of mechanical and electrical stimulation on the expression of Cx-43 mRNA is higher than that under unique tensile stimulation or electrical stimulation,which is more beneficial to the development and connection of cardiomyocytes.
基金
National Natural Science Foundation of China
grant number:10802006,10925208,11120101001 and 10972024
