肺癌细胞物质表达及堆积结构的拉曼位移特征

Raman Shift Characteristics of Substance Expression and Accumulation Structure in Lung Cancer Cells

分析了肺腺癌细胞和间皮细胞间的拉曼位移及细胞化学染色形态学特征参数间的关系,为非创伤性检查肿瘤细胞提供参考。选择A549和Met-5A两种细胞进行拉曼光谱分析,将两种细胞进行H&E和PAP染色,计算其形态学图像特征。两种细胞在1082,1155,1304cm^-1处存在拉曼位移。通过图像识别A549和Met-5A细胞的能量标准差,采用PAP染色方法的两种细胞差异具有统计学意义(p<0.05),但是采用H&E染色方法的两种细胞差异无统计学意义(p>0.05);对于颜色均值特征值,采用两种染色方法的两种细胞差异均具有统计学意义(p<0.05)。基于化学物质亲和特性,从肿瘤物质表达及堆积结构方面分析肺癌细胞拉曼位移特征,实验结果显示,临床组织病理染色分析核酸及蛋白表达特征,而拉曼光谱分析发现肺癌细胞存在核酸、蛋白表达及脂质结构的拉曼峰位移,这为拉曼检测肿瘤细胞物质波峰设定提供参考。

To lay a foundation for the non-invasive examination of tumor cells,the associations between Raman shift characteristics and morphological parameters of cytochemical staining are analyzed in lung adenocarcinoma cells and physiological mesothelial cells.A549 cells and Met-5 Acells are analyzed using Raman spectroscopy to calculate the Raman spectral shift characteristics.A549 cells and Met-5 Acells are stained with H&E and PAP solutions to calculate their morphological image characteristics.In both cell lines,Raman shifts are observed at 1082,1155,and1304 cm^-1.Energy standard deviations of A549 and Met-5 Acells are recognized by the images.PAP staining results are significantly different(p<0.05),but H&E staining results show no significant differences(p>0.05)between the cell lines.The mean color value of cell image is significantly different(p<0.05)between the two stains.The Raman shift characteristics of lung cancer cells are then analyzed in terms of tumor substance expression and accumulation structure based on the affinity characteristics of the chemical substances.Experimental results show that clinical histopathological staining reveals the characteristics of nucleic acid and protein expression,and Raman spectroscopy reveals Raman peak shifts of nucleic acids,protein expression,and the lipid structure in lung cancer cells,laying a foundation for Raman spectroscopy to be used to set the substance peak of tumor cells.