2017年河南省7株麻疹病毒分离株血凝素基因特征分析

Analysis on the hemagglutinin genetic characteristic of 7 measles virus isolated in Henan Province in 2017

目的分析河南省2017年麻疹病毒分离株的血凝素基因特征.方法咽拭子标本来源于2017年河南省麻疹监测信息系统中报告的7例麻疹实验室确诊病例,用非洲绿猴肾细胞/淋巴信号激活因子转染的非洲绿猴肾细胞(Vero/Slam)从标本中分离麻疹病毒,用逆转录PCR扩增麻疹分离株的血凝素蛋白(H)基因,再对扩增产物进行核苷酸序列测定和分析.结果 7例麻疹病例的年龄为1~50岁,均为男性.7株分离株均为H1a基因型,核苷酸、氨基酸平均遗传距离均为0.005,与Shanghai-191/China-vaccine毒株序列进行比较,氨基酸序列240、397、481位点发生氨基酸的突变.结论河南省2017年麻疹病毒流行株为H1a基因型,与Shanghai-191/China-vaccine株相比较,在基因特性上存在差异,需进一步加强对麻疹病毒变异程度的监测.

Objective Analyze the genetic characteristic of Hemagglutinin(H) gene of measles viruses isolated in Henan Province in 2017. Methods Swab samples collected from 7 lab confirmed measles cases,and we got the measles virus by Vero/Slam inoculation. Fragment of H genes were amplified by reverse transcription polymerase chain reaction(RT-PCR), then the PCR products were sequenced and analyzed. Results The age of the 7 measles confirmed cases were between 1 and 50 years old,and all of them were males. All the 7 measles viruses were identified as H1a genotype,and the average distance of the nucleotides and the amino acids was 0.005, respectively. Compared with the Shanghai-191/China-vaccine, there were some changes in isolated virus,such as 240th, 397th and 381st sites in the amino acid sequence. Conclusion The measles genotype which isolated in Henan Province in 2017 was H1a. There were some difference from Shanghai-191/China-vaccine in the nucleotides sequence of H gene,which suggested that it's necessary to strengthen the monitor the variation of measles virus.