miR-143/miR-145簇启动子区rs4705342基因突变与汉族人群前列腺癌发病的相关性研究

The rs4705342 gene mutation in the promoter region of the miR-143/145 cluster associated with the risk of prostate cancer in the Chinese Han population

目的:选择miR-143/miR-145基因启动子区rs4705342突变位点,研究其与汉族人群前列腺癌发病率的相关性。方法:156例确诊为前列腺癌的患者为病例组,188例健康男性为对照组,采用TaqMan法对miR-143/miR-145基因启动子区rs4705342进行多态性检测;并针对肿瘤家族史、体质量指数(BMI)、年龄、吸烟及饮酒等进行分层分析。结果:病例组与对照组的年龄(P=0.32),BMI(P=0.79),吸烟(P=0.47)、饮酒(P=0.34)状况未发现有统计学意义,肿瘤家族史比例病例组显著高于对照组(P=0.01)。TT/CT联合基因型与CC基因型分布无统计学意义(P=0.07)。在不吸烟亚组中,CC基因型与TT/CT联合基因型的分布具有统计学意义(P=0.02);在饮酒亚组中,CC基因型与TT/CT联合基因型的分布具有统计学意义(P=0.03);在前列腺癌临床分期中,CC基因型与TT/CT联合基因型的分布未发现明显差异(P=0.81),PSA分层分析中CC基因型与TT/CT联合基因型的分布未发现明显差异(P=0.39)。结论:miR-143/miR-145簇启动子区rs4705342基因突变与汉族人群前列腺癌发病有明显关联。

Objective:To explore the correlation between the rs4705342 gene mutation in the promoter region of the miR-143 / miR-145 cluster and the risk of PCa in the Chinese Han population.Methods:We enrolled in this study 156 cases of PCa and 188 cancer-free controls.Using TaqMan SNP genotyping assay,we detected the polymorphism of rs4705342 in the promoter region of the miR-143 / miR-145 gene,and performed a stratified analysis on the family history of cancer,body mass index (BMI),age,smoking status,and alcohol consumption of the subjects.Results:The proportion of family history of cancer was significantly higher in the case group than in the control (P = 0.01).No statistically significant differences,however,were found between the two groups in age (P = 0.32),BMI (P = 0.79),smoking status (P = 0.47),and alcohol consumption (P = 0.34),nor in the distribution of the TT,CT and CC genotypes (P = 0.07) except in that of the CC and TT/CT combined genotypes (P = 0.01).There were statistically significant differences in the distribution of CC and TT/CT combined genotypes between the non-smoking subgroups,(P = 0.02) and alcohol drinking subgroups (P = 0.03),but not between the clinical stages of PCa (P = 0.81) or the levels of PSA (P = 0.39).Conclusion:The rs4705342 gene mutation in the promoter region of the miR-143 / miR-145 cluster is significantly associated with the pathogenesis of PCa in the Chinese Han population.