去甲肾上腺素在抑郁状态下提高乳腺癌的恶性程度

Norepinephrine increases risk of breast cancer in depressed women

目的探究抑郁对肿瘤的影响及其可能的分子机制。方法构建慢性轻度不可预见性应激模型(CUMS),行为学检测后接种肿瘤,检测血清去甲肾上腺素以及肿瘤体积组间差异;给药刺激乳腺癌细胞并检测侵袭、迁移、增殖及细胞周期分布;免疫印迹及免疫荧光检测细胞表面受体及胞内信号通路。结果抑郁上调去甲肾上腺素水平,使p38MAPK的磷酸化程度增强,并导致乳腺癌细胞恶性程度增加。结论抑郁通过去甲肾上腺素激活肾上腺素β受体,促进p38MAPK信号通路磷酸化,从而增加乳腺癌的恶性程度。

Aim To explore the effects of depression on tumors,as well as its molecular mechanisms.Methods The chronic unpredictable mild stress model (CUMS) was constructed.Tumor was inoculated after behavioral test,and the differences in serum norepinephrine and tumor volume between groups were detected.Breast cancer cells were stimulated by administration and invasion,migration,proliferation and cell cycle distribution were detected.Western blot and immunofluorescence were utilized to detect cell surface receptors and intracellular signaling pathways.Results Depression up-regulated the level of norepinephrine (NE) and increased the phosphorylation of p38 MAPK,resulting in increased malignancy of breast cancer cells.Conclusions Depression may increase the malignancy of breast cancer through norepinephrine-induced p38 MAPK signaling pathway,thus increasing the malignant degree of breast cancer.