现代代谢组学平台建设及相关技术应用

Current metabolomics platforms: technical composition and applications

代谢组作为生命科学研究的5个层面(基因组、转录组、蛋白质组、代谢组和表型组)之一越来越受到科研工作者的关注。色谱-质谱联用技术由于其高分离能力、高灵敏度等优点在代谢组学(定性和定量)领域发挥重要的作用,基于色谱-质谱联用技术的代谢组学已成功应用于代谢表型差异研究、基因功能鉴定和转基因生物安全性评价等多个研究方向。本文以中国科学院遗传与发育生物学研究所代谢组学平台为例,详细介绍了现代代谢组学平台色谱-质谱联用仪器的硬件组成,以及不同技术平台在现在系统生物学研究中的具体应用。

Metabolomics (defined as comprehensive small molecule chemical analysis),together with genomics, transcriptomics,proteomics and phenomics,now plays a fundamental role in system biological studies.Chromatographymass spectrometry machines,which have the characteristics of high resolution and high sensitivity,are widely used for metabolomics analysis,both qualitatively and quantitatively.With the fast development of the chromatography-mass spectrometry technology,metabolomics analysis has been successfully applied in various biological research fields.Here, we introduce the different chromatography-mass spectrum machines used for metabolomics analysis and their applications to various biological issues by mainly using the metabolomics platform in Institute of Genetics and Developmental Biology as a case study.