联用超高效液相色谱-高分辨飞行时间质谱技术对扶正化瘀胶囊化学成分快速鉴别

Rapid identification of chemical constituents of Fuzheng Huayu capsules by UHPLC-TOF/MS

目的 运用超高效液相色谱-高分辨飞行时间质谱(UHPLC-TOF/MS)联用技术鉴别扶正化瘀胶囊中化学成分。方法 色谱分离采用ACQUITY UPLCHSS T3 柱(2.1 mm×100 mm,1.8 μm);流动相0.1%甲酸水溶液(A)-0.1%甲酸乙腈溶液(B),梯度洗脱条件:0~3 min,2%B;3~18 min,2%~50%B;18~22 min,50%~95%B;22~25 min,95%B。平衡时间为 10 min ,流速为0.4 ml/min,分析时间为25 min。质谱定性采用电喷雾离子源,正/负离子模式扫描,采集范围100~ 1 100 m/z 。结果 正/负离子模式下,一次性鉴别出154个成分,其中,正、负离子模式下均有响应的有 28个。结论 较全面地阐明了扶正化瘀胶囊的成分组成,为该复方的深入研究奠定了基础。

Objective To identify the chemical constituents in Fuzheng Huayu capsule by ultra performance liquid chromatography-high resolution time of flight mass spectrometry(UHPLC-TOF/MS). Methods ACQUITY UPLCHSS T3(2.1mm × 100mm,1.8μm) was used to chromatographic separation;mobile phase 0.1% formic acid aqueous solution(A)-0.1% formic acid acetonitrile solution(B),gradient elution conditions:0-3min,2% B;3-18 min,2%-50% B;18-22 min,50%-95% B;22-25 min,95% B.The equilibration time was 10 min,the flow rate was 0.40 ml/min,and the analysis time was 25 min .Mass spectrometry was performed by an electrospray ion source using a positive-negative ion mode scan with a range of 100-1 100 m/z . Results 154 components were identified at one time,of which 28 were positive and negative ion modes. Conclusion The composition of Fuzheng Huayu capsule was comprehensively clarified in this study,which laid a foundation for further research of this compound.