用气相色谱法测定血液制品生产过程中中间品乙醇的含量

Determination of ethanol content in intermediate products during the production of blood products by GC

目的 建立气相色谱法测定血液制品生产过程中中间品乙醇含量的方法。方法 采用DB-624毛细管色谱柱,FID检测器,气化室温度 230℃;检测器温度 280℃;柱温:40℃,保持5 min后,以10 ℃/min升温至80℃,再以50 ℃/min升温至230℃,保持3 min;以正丙醇为内标。结果 乙醇在0.5%~5%浓度范围内线性关系良好( r =1.000 0),平均回收率为99.28%,RSD为0.01%( n =9)。结论 该方法简单灵敏,结果准确可靠,可用于血液制品生产过程中对中间品乙醇含量的测定。

Objective To establish a method for determination of ethanol content in intermediate products during the production of blood products by GC. Methods DB-624 column was used with FID detection.The temperature of the injector and detector was 230℃,280℃,respectively.The column temperature was kept at 40℃ for 5 minutes,then increased to 80℃ at 10℃ per minute,and finally to 230℃ at 50℃ per minute for 3 minutes.N-propanol was used as the internal standard. Results The standard curve was linear in the range of 0.05%-5 %( r =1.000 0) for ethanol.The average recovery was 99.28% with RSD of 0.01%( n =9). Conclusion This method was simple,sensitive,accurate and reliable.It is suitable for the determination of ethanol content in intermediate products during the production of blood products.