大花蕙兰花粉贮藏与离体萌发研究

Study on preservation and germination of Cymbidium hybridum pollen

为解决兰花花期不遇的问题,为杂交育种工作提供参考与借鉴。以大花蕙兰(Cymbidiumhybridum)品种JOIC和KK560为试材,筛选适宜的花粉离体萌发培养基配方;检测不同贮藏温度、不同保存时间的大花蕙兰花粉离体萌发率,确定适宜的花粉保存方法。结果显示,大花蕙兰品种JOIC花粉粒离体萌发的最适培养基配方为0.5MS+30%蔗糖,大花蕙兰品种KK560花粉粒离体萌发的最适培养基配方为0.01%硼酸+0.2%氯化钙+30%蔗糖。建议保存前将花粉块硅胶阴干24h,-20℃保存,期限为6个月。

To solve the problem of the flowering worst of orchid and provide reference for orchid hybridization breeding. This study have used the Cymbidium hybridum species JOIC and KK560 as test materials to screen the optimal medium for in vitro pollen germination,and detected the in vitro germination rate of Cymbidium hybridum pollen with different storage temperature and storage time to determine the appropriate pollen preservation method. The results showed that the optimum medium for in vitro germination of JOIC pollen was 0.5 MS+30% sucrose,and the optimum medium for in vitro germination of KK560 pollen was 0.01% H3BO3+0.2% CaCl2+30% sucrose. Pollinium should be dried in the shade with silicone for24 h before preservation and be preserved in -20 ℃ for six months.