生物芯片技术检测奶牛饲料中多种真菌毒素

Detection of Multiple Mycotoxins in Dairy Feeds Using Biochip Technology

利用生物芯片真菌毒素阵列,选用其中的赭曲霉毒素A、脱氧雪腐镰刀菌烯醇、黄曲霉毒素B1和玉米赤霉烯酮4个组分,同时测定其在奶牛饲料中的残留量,考察该生物芯片的准确性、精密度和重现性等指标.结果表明:4种真菌毒素的标准曲线线性相关系数均可达到0.99以上;试剂盒质控样品和阴性样品2个水平的加标回收率在80%~120%之间,变异系数(coefficient of variance,CV)在15%以内,方法准确性和重复性较好;对奶牛饲料样品检测结果重现性考察的CV在10%以内,表明方法重现性较好;与高效液相色谱法相比,2种检测方法的结果差异性小,且生物芯片法前处理更为简便.真菌毒素阵列生物芯片法操作简单、结果准确,缩短了大量样本的筛查时间,为批量样品筛查提供了可靠的技术保证.

The residues of ochratoxin A, deoxynivalenol, aflatoxins B1 and zearalenone in dairy feeds were simultaneously detected using biochip array technology. The method accuracy, precision and reproducibility were evaluated. The results obtained that the correlation coefficient of the calibration curve for each analyte was greater than 0.99. The recoveries for quality control sample and negative sample at two spiked levels varied from 80% to 120%. The coefficient of variance (CV) was less than 15% for accuracy and reproducibility and was less than 10% for reproducibility. Compared with high performance liquid chromatography, this method gave consistent results with a simpler sample pretreatment procedure. In conclusion, the biochip array method was simple and accurate and it could provide an efficient and reliable tool for mass screening of samples.