VKORC1基因rs9923231及GGCX基因rs2592551位点多态性与新疆维吾尔族、汉族人群心源性脑栓塞的相关性研究

Association of VKORC1 gene rs9923231, GGCX Gene rs2592551 Polymorphism with the Cardiogenic Cerebral Embolism in Xinjiang Uygur and Han Population

目的:探讨维生素K环氧化物还原酶复合体亚单位1(VKORC1)基因rs9923231及γ-谷氨酰羧化酶(GGCX)基因rs2592551位点多态性同新疆维吾尔族和汉族人群发生心源性脑栓塞的关系。方法:选取2017年1月至2018年10月曾就诊于新疆医科大学第六附属医院神经内科的维吾尔族和汉族散发性房颤致脑栓塞患者各50例做为病例组,同时选取非心源性脑卒中维吾尔族患者50名及汉族患者150名为对照组,从外周静脉血中提取基因组DNA,采用PCR-RFLP技术检测VKORC1基因rs9923231及GGCX基因rs2592551多态性位点在不同人群中的分布。结果:维吾尔族脑栓塞组及对照组VKORC1基因rs9923231多态性位点基因型频率分布差异有统计学意义(P<0.05);汉族脑栓塞组及对照组VKORC1基因rs9923231多态性位点基因型频率的分布比较差异无统计学意义(P>0.05)。GGCX基因rs2592551多态位点在汉族、维吾尔族脑栓塞组及对照组的分布差异均无统计学意义(P>0.05)。结论:VKORC1基因rs9923231多态性可能与新疆维吾尔族人群心源性脑栓塞的发病有关,而与汉族人群无关;GGCX基因rs2592551多态性可能与维吾尔族及汉族人群心源性脑栓塞的发病均无关。

Objective: To explore the association between vitamin K epoxide reductase complex subunit 1(VKORC1) gene rs9923231 and γ-glutamyl carboxylase(GGCX) gene rs2592551 polymorphism and cardiogenic cerebral embolism in Xinjiang Uygur and Han populations. Methods: 50 patients with cerebral embolism caused by sporadic atrial fibrillation in Uygur and Han nationality who had been treated in the Department of Neurology, the Sixth Affiliated Hospital of Xinjiang Medical University from January 2017 to October 2018 as a case group. At the same time, 50 non-cardiac stroke Uygur patients and 150 Han patients were selected as the control group. Genomic DNA was extracted from peripheral venous blood, and the distribution of VKORC1 gene rs9923231 and GGCX gene rs2592551 polymorphisms in different populations was detected by PCR-RFLP technique. Results: The frequency distribution of VKORC1 gene rs9923231 polymorphism in Uygur cerebral embolism group and control group was statistically significant(P<0.05);Han nationality cerebral embolism group and control group VKORC1 gene rs9923231 polymorphism gene There was no significant difference in the distribution of type frequency(P>0.05). There was no significant difference in the distribution of GGCX gene rs2592551 polymorphism in Han and Uygur cerebral embolism group and control group(P>0.05). Conclusion: The VKORC1 gene rs9923231 polymorphism may be associated with the onset of cardiogenic cerebral embolism in Xinjiang Uygur population, but not related to the incidence of cerebral embolism in Han population;The GGCX gene rs2592551 polymorphism may not be associated with the onset of cardiogenic cerebral embolism in Uygur and Han populations.