葡萄糖对小鼠CD4^+ T细胞分化的影响和机制初探

Primary study on the effect of glucose on mouse CD4^+ T cell differentiation and its mechanism

目的·探讨葡萄糖对小鼠CD4^+T细胞分化的影响。方法·小鼠初始CD4^+T细胞在调节性T细胞(regulatory T cell,Treg)、Th1、Th17和Th2分化条件下,经不同浓度的葡萄糖处理5 d后,利用流式细胞术检测细胞分化的比例,实时荧光定量PCR检测相关细胞因子和转录因子的基因表达水平。结果·与无糖组相比,在Treg和Th2分化的条件下,随着葡萄糖浓度升高,Treg和Th2比例增高,其关键转录因子Foxp3(forkhead box P3)、Gata3(GATA binding protein 3)与主要细胞因子转化生长因子-β、白介素-4(interleukin-4,IL-4)和IL-13的基因水平增加;而随着葡萄糖浓度升高,Th1和Th17细胞比例则降低,其相关转录因子Tbx21(T-box transcription factor 21)和RORC(RAR related orphan receptor C),以及细胞因子干扰素-γ、IL-17A、IL-17F、IL-22和细胞因子受体IL-23R的基因表达下降。结论·葡萄糖能促进Treg和Th2体外分化,而抑制Th1与Th17分化。

Objective·To study the effect of glucose on mouse CD4^+T cell differentiation.Methods·Mouse na?ve CD4^+T cells cultured in the regulatory T cell(Treg),Th1,Th17 or Th2 differention condition were treated with different concentrations of glucose for 5 days.Treg,Th1,Th17 or Th2 percentages were measured by flow cytometry.Quantitative real-time PCR was used to detect the gene expressions of related cytokines and transcriptional factors.Results·The proportions of Treg and Th2 as well as the gene expressions of transforming growth factor-β,interleukin-4(IL-4)and IL-13,and transcriptional factors,Foxp3(forkhead box P3)and Gata3(GATA binding protein 3),were increased significantly with the treatment of increasing concentration of glucose.On the contrary,with the glucose treatment,the percentages of Th1 and Th17 were reduced,and the gene expressions of the related cytokines and cytokine receptors,such as interferon-γ,IL-17A,IL-17F,IL-22 and IL-23R,and the related transcriptional factors,Tbx21(T-box transcription factor 21)and RORC(RAR related orphan receptor C),were decreased consistently.Conclusion·Glucose promotes Treg and Th2 differentiation while inhibits Th1 and Th17 differentiation in vitro.