640nm红光通过CD26促进角质形成细胞迁移的实验研究

Promotion of 640 nm red light on keratinocyte migration via CD26

目的·初步探究640 nm红光促进角质形成细胞迁移的机制。方法·体外培养人角质形成细胞,根据干预方式不同分为4组:对照组、sitagliptin组(抑制CD26表达)、640 nm红光组(8 J/cm2)、640 nm红光+sitagliptin组。Realtime-PCR检测角质形成细胞中CD26 mRNA的表达水平,Western blotting检测CD26蛋白表达情况,Transwell和细胞划痕实验观察角质形成细胞的迁移能力。结果·Realtime-PCR和Western blotting结果显示,640 nm红光组与对照组相比CD26 mRNA和蛋白表达有所升高,而sitagliptin组明显低于对照组(均P<0.05);Transwell和细胞划痕实验均可观察到640 nm红光组角质形成细胞迁移能力增强,而sitagliptin组迁移能力下降。结论·640 nm红光照射可促使CD26表达增加,加快角质形成细胞迁移,从而缩短再上皮化进程。

Objective·To investigate the mechanism of 640 nm red light promoting keratinocyte migration preliminarily.Methods·Human keratinocytes were cultured in vitro and assigned to 4 groups according to different interventions,i.e.control group,sitagliptin group(CD26 expression inhibited),640 nm red light group(8 J/cm2)and 640 nm red light+sitagliptin group.The levels of CD26 mRNA and protein in keratinocytes were measured with realtime-PCR and Western blotting,respectively.Cell migration was observed with Transwell assay and scratch assay.Results·The level of CD26 mRNA and the expression of CD26 protein in 640 nm red light group obviously increased compared with control group,while those of sitagliptin group significantly decreased(all P<0.05).Migration ability of keratinocytes increased in 640 nm red light group and decreased in sitagliptin group observed by Transwell assay and scratch assay.Conclusion·The expression of CD26 in keratinocytes is promoted by the 640 nm red light,which can accelerate keratinocyte migration to facilitate re-epithelialization.