蚯蚓肽对拟南芥生长和细胞质Ca2+浓度的影响

Effect of earthworm peptide on Arabidopsis growth and the concentration of Ca2+ in the cytoplasm

为了探讨蚯蚓肽对拟南芥生长及细胞质Ca2+浓度的影响,选取9种蚯蚓肽添加到拟南芥生长培养基中,观察其对拟南芥生长的影响。结果表明:蚯蚓肽Lumbricin-PG-27、AVPF、VQ-5、F-1和LumA5对拟南芥生长具有显著促进作用,其中LumA5的促进作用最强。以9种蚯蚓肽溶液处理稳定表达水母发光蛋白基因的拟南芥幼苗,发现蚯蚓肽LumA5可以使拟南芥细胞质钙离子浓度[Ca2+]cyt升高,添加抑制剂Alloxan、GdCl3、Neomycin可显著抑制LumA5引起的细胞质钙离子浓度[Ca2+]cyt的升高,这一发现为蚯蚓肽应用于农业生产中提供了科学依据。

To investigate the effect of earthworm peptide on Arabidopsis growth,nine earthworm peptides were tested.The results showed that Lumbricin-PG-27,AVPF,VQ-5,F-1,and especially LumA5 had a significant enhancing effect on Arabidopsis growth.An increased[Ca2+]cyt was found in Arabidopsis seedlings treated with LumA5.The results also showed that the Alloxan,GdCl3,and Neomycin can inhibit the increase of [Ca2+]cyt treated with LumA5.This study provides scientific evidence for the application of earthworm peptides in agricultural production.