摘要
目的研究人和动物C组轮状病毒VP8^*蛋白与糖的结合特征,为人和动物C组轮状病毒感染机制提供基础和依据.方法利用原核表达系统表达并纯化人和动物病毒毒株的VP8^*蛋白,通过糖点阵实验、寡糖结合实验及血凝实验等方法研究其糖结合特征.结果人C组轮状病毒sz94 VP8^*蛋白不仅与A糖结合,还与黏蛋白核心mucin core 4糖结合.狗C组轮状病毒糖点阵实验显示,可以结合A糖,人和狗C组轮状病毒可以凝集A型红细胞,猪P[5]、P[8]、P[19]、P[21]与实验中的寡糖都没有结合,也都没有凝集各型红细胞.结论黏蛋白核心在人C组轮状病毒可能是潜在的粘附分子,A型组织血型抗原可能也是某些动物C组轮状病毒的粘附分子,猪C组轮状病毒具体结合的糖类型还需进一步探索.
Objective To explore the glycan binding specificity of VP8^* protein of human and some animal group C rotavirus (RVC), so as to provide certain basis and evidence for illustrating the mechanism of human and animal RVC infection and epidemiology. Methods The VP8^* proteins of human and animal RVC were expressed with prokaryotic expression system and were purified. Interactions between RVC VP8^* proteins and glycans were explored by the glycan microarray assay, oligosaccharide binding assay and hemagglutination assay. Results Human RVC sz94 VP8^* protein bound to type A glycan, and mucin core 4 oligosaccharide. Dog RVC VP8* showed binding to type A glycan in the glycan microarray assay. Human RVC and dog RVC hemagglutinated type A blood cells. Porcine RVCs P[5], P[8], P[19] and P[21] VP8^*s showed no binding to all tested oligosaccharides and no hemagglutination on all types of blood cells. Conclusions Mucin core maybe a potential attachment factor of human RVC while type A glycans may be potential cell attachment factors for some animal RVCs. Glycan binding patterns of procine RVC need further exploration.
作者
党蕾
孙晓曼
李丹地
高筱惠
黄秋玉
白瑞霞
段招军
Dang Lei;Sun Xiaoman;Li Dandi;Gao Xiaohui;Huang Qiuyu;Bai Ruixia;Duan Zhaojun(Inner Mongolia Clinical Medical College, Inner Mongolia Medical University, Hohhot 010059, China;Key Laboratory of Medical Viruses and Viral Diseases, National Health Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Diseases Control and Prevention, Beijing 102206, China;Department of Clinical Laboratory, Inner Mongolia People’s Hospital, Hohhot 010017, China)
出处
《国际病毒学杂志》
2019年第4期220-224,共5页
International Journal of Virology
基金
国家科技重大专项(2018ZX10711-001)
国家自然科学基金(81601813)
国家重点研发计划(2018YFC1200602).
关键词
C组轮状病毒
VP8^*蛋白
糖结合特征
寡糖结合实验
血凝实验
Group C rotavirus
VP8^* protein
Glycan binding specificity
Oligosaccharides binding assay
Hemagglutination assay