反相超高效液相色谱法同时测定植物油中8种V_E异构体

Simultaneous determination of 8 kinds of vitamin E isomer in vegetable oil using reversed-phase ultra performance liquid chromatography

建立了溶剂稀释-超高效液相色谱法检测植物油中8种VE异构体(生育酚及生育三烯酚)的方法。样品经丙酮溶解稀释后,以PFP色谱柱分析,甲醇-水(体积比93:7)为流动相,在0.4mL/min流速下等度洗脱,选择激发波长295nm,发射波长330nm进行检测。8种VE异构体的加标回收率为90.0%~100.0%,变异系数为0.28%~1.22%,线性范围为0.4~40.0μg/mL,相关系数>0.999,各VE异构体的方法检出限均为4.0 mg/kg。该方法检测流程简便,净化效果好,灵敏度高,可满足实际样品分析的需求。

An analytical method was developed for the determination of 8 kinds of vitamin E isomer(tocopherol and tocotrienol)in vegetable oil by solvent dilution extraction-ultra performance liquid chromatography.The sample was extracted with acetone.The separation was achieved by using PFP column and an elution with the mobile phases of methanol and water(93:7)at a flow rate of 0.4 mL/min.The identification and quantitative analysis were carried out with fluorescence detector at an ecxitation wavelength of 295 nm and an emission wavelength of 330 nm.The recoveries ranged from 90.0%to 100%,with the relative standard deviation(RSDs)between 0.28% and 1.22%.The linear range was 0.4~40.0μg/mL and the correlation coefficient(r)was greater than 0.999.The limits of detection were 4.0 mg/kg for each vitamin E isomer.The method was enough for the analysis of vitamin E in actual samples with wide linear range and high sensitivity.