HPLC法同时测定刺玫果提取物中的黄酮类成分及其体外活性测定

Simultaneous determination of flavonoids in fruits of Rosa davurica pall. by HPLC and measurement of their activities in vitro

采用HPLC法同时测定刺玫果提取物中4种黄酮类成分的含量,并采用UV法测定刺玫果提取物、4种黄酮类单体和单体复方配伍的体外活性。结果表明,刺玫果提取物中金丝桃苷、芦丁、槲皮素及木犀草素的含量分别为607.98,450.63,187.32,12.68μg/g。刺玫果提取物、4种黄酮类成分及其复方配伍均具有一定的体外抗氧化活性,且刺玫果提取物的活性强于黄酮类成分的复方配伍,但二者均弱于VC;刺玫果提取物、4种黄酮类成分及其复方配伍均具有清除亚硝酸盐、阻断亚硝胺合成及抑制α-糖苷酶的活性,且刺玫果提取物的活性强于阿卡波糖、黄酮类成分的复方配伍。

Simultaneous determination of four flavonoids in extracts of the fruits of Rosa davurica pall.(FRDP)by high performance liquid chromatography(HPLC)was carried out,and the in vitro activities of extracts of FRDP,four flavonoids and their compound compatibility were measured by UV method.The results showed that the contents of hyperoside,rutin,quercetin and luteolin of the extract of FRDP were 607.98,450.63,187.32 and 12.68μg/g respectively.The extracts of FRDP,four flavonoids and their compound compatibility all have certain antioxidant activities in vitro,and the activity of extracts of FRDP was stronger than that of compound compatibility.However,both of them were weaker than that of VC.The extracts of FRDP,fourflavonoids and their compound compatibility also had the activity of scavenging nitrite,blocking the synthesis of nitrosamine and inhibitingα-glucosidase,and the activity of extract of FRDP was stronger than those of acarbose and compound compatibility.