红景天苷对小鼠抗血小板活性和抗血栓作用

Experimental Study on Aantithrombotic Effect of Salidroside

目的:探讨红景天苷(Sal)对小鼠的抗血小板活性及抗血栓作用。方法: 120只小鼠随机均分为对照组(生理盐水)、阳性药组(阿司匹林100 mg/kg)、Sal低剂量组(50 mg/kg)和Sal高剂量组(100 mg/kg),雌雄各半,各组每只小鼠腹腔注射相应试剂1次/d,连续7 d;末次给药30 min时取血检测小鼠白细胞(WBC)、红细胞(RBC)、血小板计数(PLT)、平均血小板体积(MPV),凝血功能4项指标凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、凝血酶时间(TT)及纤维蛋白原(FIB),采用断尾流血法检测小鼠断尾出血时间;40只雄性小鼠按上述分组方式,每组10只,腹腔注射1次/d,第5天给药30 min后腹腔注射角叉菜胶(20 mg/kg,0.1 mL/10 g),第6天、第7天持续给予Sal,分别测量并记录角叉菜胶12、24、48 h后的黑尾率以及黑尾长度。结果:各组小鼠WBC、RBC、PLT和MPV差异均无统计学意义( P> 0.05);与对照组小鼠相比,Sal高、低剂量组小鼠PT、APTT、TT及FIB差异均无统计学意义( P >0.05);阳性药组、Sal低剂量组和Sal高剂量组小鼠断尾平均出血时间分别较对照组为长( P <0.05或 P <0.01);造模12 h时,对照组小鼠黑尾率明显高于Sal高剂量组小鼠( P <0.01);造模24 h时,对照组小鼠黑尾率明显高于阳性药组和Sal高剂量组小鼠( P <0.05);在造模12 h、24 h和48 h时,与对照组小鼠相比,阳性药组、Sal低剂量组和Sal高剂量组小鼠黑尾长度均有缩短,差异有统计学意义( P <0.05或 P <0.01)。结论: Sal对血栓形成具有明显的抑制作用,其机制可能与抗血小板活性有关。

Objective: To explore the anti-platelet activity and anti-thrombotic effect of salidroside (Sal) on mice. Methods: 120 mice were randomly divided into the control group (normal saline), the the positive drug group (aspirin,100 mg/kg), Sal low-dose group (50 mg/kg) and Sal high-dose group (100 mg/kg), with half male and half female. Each mouse of the groups was intraperitoneally injected with corresponding reagent once per day for 7 days. Blood samples were taken in 30 min after the last administration to detect white blood cells (WBC), red blood cells (RBC), platelet count (PLT), mean platelet volume (MPV), four indexes of coagulation function, prothrombin time (PT), activated partial thrombin time (APTT), thrombin time (TT) and fibrinogen (FIB), and the bleeding time of mouse tail was detected by tail-breaking bleeding method. 40 male mice were grouped as above , 10 in each group, with intraperitoneal injection once per day, then intraperitoneal injection of carrageenan in 30 min after the administration on the 5th day,(20 mg/kg, 0.1 mL/10 g). Sal was continuously given on the 6th and 7th days, and the black tail rate and the black tail length of carra- geenan 12 h, 24 h, and 48 h later were measured and recorded. Results: No significant difference was found in WBC, RBC, PLT and MPV in all groups ( P >0.05). Compared with the control group, there was no significant difference in PT, APTT, TT and FIB in both Sal high and low dose groups ( P >0.05). The average bleeding time in the positive drug group, Sal low-dose group and Sal high-dose group was longer than that in the control group respectively ( P <0.05 or P <0.01). The black tail rate of the control group was significantly higher than that of Sal high dose group ( P <0.01) 12 h after modeling. The black tail rate of the control group was significantly higher than that of positive drug group and Sal high-dose group ( P <0.05) 24 h after modeling. In 12 h, 24 h and 48 h after modeling, the black tail length of the positive drug group, Sal low-dose group and Sal high-dose group was shortened compared with the control group, and the difference was statistically significant ( P <0.05 or P <0.01). Conclusion: Sal has obvious inhibitory effect on thrombosis, and the mechanism may be related to antiplatelet activity.