固相萃取-高效液相色谱法测定润肠合剂中大黄的五种成分的含量

Determination of Five Components of Rhubarb in Runchang Mixture by Solid Phase Extraction-High Performance Liquid Chromatography

目的建立润肠合剂中大黄五种成分含量的测定方法,为药品质量控制提供依据参考。方法精密称取用以配制润肠合剂的大黄1.0 g制成供试品溶液,采用固相萃取技术,以高效液相色谱仪测定润肠合剂中大黄的五种蒽醌类成分大黄素、大黄酸、大黄酚、芦荟大黄素、大黄素甲醚的含量,色谱柱Lichrospher-C18柱(4.6×250 mm,5μm)。结果各成分均有与峰面积呈良好线性关系的浓度范围,r=0.999 9、1.000 0、0.999 7、0.999 8、1.000 0。各成分平均回收率分别为99.88%、99.97%、100.06%、100.01%、99.98%,RSD分别为0.76%、1.03%、0.55%、2.14%、1.96%。结论固相萃取-高效液相色谱法测定润肠合剂中大黄五种成分的含量简单易行、准确可靠,可用于润肠合剂质量控制。

Objective To establish a method for the determination of five components of rhubarb in Runchang Mixture,and to provide a basis for drug quality control.Methods 1.0 g of rhubarb used to prepare Runchang Mixture was accurately prepared for the test solution.Solid phase extraction technique was used to determine the five steroidal components of emodin in rhubarb.The content of rhein,chrysophanol,aloe-emodin,emodin methyl ether,column Lichrospher-C18 column (4.6 × 250 mm,5 μm).Results Each component had a concentration range with a good linear relationship with the peak area,and r was equal to 0.999 9,1.000 0,0.999 7,0.999 8,and 1.000 0,respectively.The average recoveries of the components were 99.88%,99.97%,100.06%,100.01%,and 99.98%,respectively,and the RSD was 0.76%,1.03%,0.55%,2.14%,and 1.96%,respectively.Conclusion Solid phase extraction-high performance liquid chromatography (HPLC-HPLC) is a simple,accurate and reliable method for the determination of five components of rhubarb in Runchang Mixture.It can be used for quality control of Runchang Mixture.