反复自然流产患者检测抗心磷脂抗体以及抗β2糖蛋白Ⅰ抗体的临床意义

Clinical significance of anti-cardiolipin antibody and anti-β2 glycoprotein Ⅰ antibody in patients with recurrent spontaneous abortion

目的探讨反复自然流产(RSA)患者检测抗心磷脂抗体(ACA)以及抗β2糖蛋白Ⅰ抗体(抗β2-GPⅠ)的临床意义。方法选取2016年1月~2018年6月江西省妇幼保健院收治的117例RSA患者以及同期的60例健康经产妇(对照组)作为研究对象,根据发生自然流产时的孕龄将RSA患者分为早期流产组(65例)与晚期流产组(52例),前者孕龄<12周,后者孕龄≥12周。所有研究对象均空腹采集静脉血采用酶联免疫吸附试验(ELISA)进行ACAIgG、IgM,抗β2-GPⅠIgG、IgM检测。结果三组的ACAIgG、IgM阳性率比较,差异有统计学意义(P<0.05);晚期流产组与早期流产组的ACAIgG、IgM阳性率均高于对照组,差异有统计学意义(P<0.05);晚期流产组与早期流产组的ACAIgG、IgM阳性率比较,差异无统计学意义(P>0.05);三组的抗β2-GPⅠIgG阳性率比较,差异有统计学意义(P<0.05),抗β2-GPⅠIgM阳性率比较,差异无统计学意义(P>0.05);晚期流产组与早期流产组的抗β2-GPⅠIgG阳性率均高于对照组,差异有统计学意义(P<0.05);晚期流产组与早期流产组的抗β2-GPⅠIgG阳性率比较,差异无统计学意义(P>0.05)。结论ACAIgG、ACAIgM、抗β2-GPⅠIgG可能与RSA的发生密切相关,可以用于RSA的辅助诊断。

Objective To investigate the clinical significance of anti-cardiolipin antibody (ACA) and anti-β2 glycoprotein Ⅰ antibody(anti-β2 GPⅠ) in patients with recurrent spontaneous abortion (RSA). Methods A total of 117 cases with RSA who were treated in Jiangxi Maternal and Child Health Hospital from January 2016 to June 2018 and 60 cases of healthy postpartum women (control group) during the same period were selected as the objects of the study. According to the gestational weeks of spontaneous abortion, these RSA patients were divided into early abortion group (65 cases) and late abortion group (52 cases), and the former was less than 12 weeks, and the latter was more than or equal to 12 weeks. Then the fasting venous blood samples of all subjects were collected and the ACA IgG and IgM, anti-β2 GPⅠ IgG and IgM were detected by enzyme-linked immunosorbent assay (ELISA). Results The positive rates of ACA IgG and IgM in the three groups were significantly different (P<0.05). The positive rates of ACA IgG and IgM in late abortion group and early abortion group were higher than those in control group, and the differences were statistically significant (P<0.05). There were no significant differences in the positive rates of ACA IgG and IgM between late abortion group and early abortion group (P>0.05). The positive rate of anti-β2 GPⅠ IgG in the three groups were significantly different (P<0.05), but there was no significant difference in the positive rate of anti-β2 GPⅠ IgM (P>0.05). The positive rate of anti-β2 GPⅠ IgG in late abortion group and early abortion group was higher than that in control group, and the difference was statistically significant (P<0.05). There was no significant difference in the positive rate of anti-β2 GPⅠ IgG between late abortion group and early abortion group (P>0.05). Conclusion The ACA IgG, ACA IgM and anti-β2 GPⅠ IgG may be closely related to RSA occurrence and can be used for assistant diagnosis of RSA.