益肺饮联合CIK细胞对肺癌A549细胞免疫逃逸干预的研究

Study on Intervention of Yifei Decoction Combined with CIK Cells in Immune Escape of Lung Cancer A549 Cells

目的通过对肺癌细胞增殖和凋亡的分析以及肺癌细胞TGF-β、VEGF的表达情况,从分子水平上观察益肺饮和CIK细胞联合应用时对肺癌细胞免疫逃逸的影响。方法将肺癌细胞分为6组进行干预,A:10%FBS组、B:无药血清组、C:CIK+无药血清组、D:益肺饮组、E:CIK组、F:益肺饮+CIK组。采用CCK-8法、流式细胞仪检测A549细胞的增殖、凋亡情况,运用ELISA法检测A549细胞TGF-β、VEGF的表达情况。结果(1)与A、B组比较,益肺饮和CIK单用或联用时有明显的增殖抑制、诱导细胞凋亡的作用(P<0.05);E组与F组比较,肺癌细胞的增殖、凋亡情况无明显差异(P>0.05)。(2)相比于A、B组,D、E、F组对TGF-β、VEGF的分泌均有抑制作用(P<0.05)。与D、E组比较,F组对TGF-β的抑制效果最明显(P<0.05),但VEGF的表达情况无明显差异(P>0.05)。结论益肺饮与CIK细胞联合应用能增强对肺癌细胞TGF-β的抑制效果,但对VEGF的抑制效果无明显增强作用;不能增强对A549细胞增值抑制和诱导凋亡的作用。

Objective To observe the effects of Yifei Decoction combined with CIK cells in immune escape of lung cancer cells at molecular level,through analysis of proliferation and apoptosis of lung cancer cells and expressions of transforming growth factor-β(TGF-β) and VEGF in the lung cancer cells.Methods The lung cancer cells were divided into 6 groups for intervention.A: 10% FBS group,B: drug-free serum group,C: CIK + drug-free serum group,D: Yifei Decoction group,E: CIK group,and F: Yifei Decoction + CIK group.The proliferation and apoptosis of A549 cells were detected by CCK-8 method and flow cytometry.ELISA was used to detect the expressions of TGF-β and VEGF in A549 cells.Results (1) Compared with the group A and the group B,single use of Yifei Decoction or CIK,or their combination showed significant effects of inhibition on proliferation and induction of apoptosis (P<0.05).There was no significant difference in proliferation and apoptosis of lung cancer cells between the group E and the group F (P>0.05).(2) Compared with the groups A and B,there was an effect of inhibition on the secretion of TGF-β and VEGF in the groups D,E and F (P<0.05).Compared with the groups D and E,there was the most obvious inhibitory effect on TGF-β in the group F (P<0.05),but there was no significant difference in the expression of VEGF (P>0.05).Conclusion Yifei Decoction can enhance the inhibitory effect on TGF-β in lung cancer cells when combined with CIK cells,but it has no obvious enhancement effect on the inhibition of VEGF.And it can not enhance the inhibition of proliferation and induction of apoptosis in A549 cells.