酪丁酸梭菌清除饲料中玉米赤霉烯酮的能力

Remove zearalenone in fodder by Clostridium tyrobutyricum

研究酪丁酸梭菌吸附清除饲料中玉米赤霉烯酮的能力,为其在农作物饲料中的脱毒作用奠定基础。首先通过单因素实验确定初始菌质量浓度、pH、温度和时间的影响,并探究热、酸处理后的菌株清除玉米赤霉烯酮的能力,再采用微生物适应性进化方法驯化酪丁酸梭菌,以提高其脱毒效果。结果表明:当初始质量浓度为1.0×10^11个/mL的原始菌悬于pH4.0的培养基,37℃孵育24h时,对玉米赤霉烯酮毒素的清除效果分别为活菌组31.9%、热处理组44.2%、酸处理组50.2%,说明原始菌对玉米赤霉烯酮具有一定的清除能力。而驯化后的菌株经过热、酸处理,经验证当菌体密度为1.0×10^11个/mL时,酸处理后的驯化菌株对毒素的吸附效果最好,吸附率可达98.5%,有望被运用于实际生产中,从而减少饲料中的毒素含量,有利于家禽体内毒素的清除,降低毒素积累。

Zearalenone in fodder is toxic.Terefore,we used Clostridium tyrobutyricum to remove zearalenone in fodder,First,we determined the initial bacterial concentration,pH,temperature and time by single factor experiments to remove zearalenone.Then,we used microbial adaptive evolution method to domesticate Clostridium tyrobutyricum for improved detoxification effect.When the initial bacteria concentration of 1.0×10^11 was resuspended in the medium (pH 4.0) and incubated at 37 ℃ for 24 h,a better effect on removing zearalenone toxin was observed. The zearalenone clearance rate of original strain was 31.9%,the heat treatment group was 44.2% and the acid treatmet group was 50.2%,respective indicating that the origicat strain had certain ability to scaverge zearlenone. The domesticated strain treated with heat and acid could removre 98.5% zearalenone in fodder.Our findings facilitate the elimination of toxins in feed for poultry.