静电纺纳米纤维制备及其在电子生物传感器件的应用

Application of nanofiber electrode made by used nano laser pulse processing and electrospun technique to biosensing deviceds

使用简易方法直接制备可折叠的纳米纤维石墨烯薄膜电极,并且应用于电子生物传感器件的检测。以网版印刷技术制作出的石墨烯薄膜,用波长为355 nm的紫外光纳秒激光脉冲进行烧蚀加工制作出石墨烯薄膜电极。控制激光脉冲的能量密度与材料移除重叠率两个工作参数。在常压环境下,可在薄膜上制作电极间距宽为60μm的网印石墨烯薄膜电极。结合静电纺技术,使用质量分数为9%的聚乙烯醇(polyvinyl alcohol, PVA)与葡萄糖氧化酶(glucose oxidase, GOD)复合纳米纤维进行交联处理,然后再与聚二氧乙基噻吩:聚苯乙烯磺酸(poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) PEDOT:PSS)溶液进行混纺后,经由静电纺喷涂制作出可折叠的纳米纤维结构于石墨烯薄膜电极上。此外,藉由滴入不同浓度的葡萄糖溶液于石墨烯电极结构的纳米纤维传感区域内;观察其电性变化情形,测得出葡萄糖浓度在0.01~3.15 mmol/L区间范围内具有一趋近线性的变化,这检测结果将是能应用在低浓度、高灵敏性的电子生物传感器件。

The aim of this study is to fabricate foldable nanofiber graphene film electrodes directly by simple method and apply them to the detection of electronic biosensors. Graphene thin films were fabricated by screen printing and graphene electrodes were fabricated by ablation of graphene thin film with 355 nm ultraviolet nanosecond laser pulse. The working parameters, fluence of laser pulse and material removal overlap rate are controlled. In a normal atmospheric pressure, the screen-printed graphene electrode in an electrode gap of 60 μm can be made on the thin film. The polyvinyl alcohol(PVA) and glucose oxidase(GOD) composite nanofiber with mass fraction concentration of 9% and electrospun technique are used for a cross linking process. Next, it is blended with a poly(3,4-ethylenedioxythiophene:poly(styrene sulfonate)(PEDOT:PSS) solution. By electrospun method the foldable nanofiber structure is made on the graphene electrode. In the nanofiber sensing region of the graphene electrode structure drip into with glucose solution at different concentrations is observed, and an approximately linear variation is measured in the glucose concentration range of 0.01 to 3.15 mmol/L. This result has a good application for low concentration glucose inspection and it can be used for highly sensitive electronic bio-sensing devices.