非标记定量方法分析雌雄同株黄连木花芽分化关键期的差异蛋白

Differential Proteomics Analysis on Flower Buds of Monoecious Pistacia chinensis in Sex Differentiation Phases by Label-free LC-MS/MS

为确定雌雄同株黄连木两个花芽分化关键期中差异化表达的蛋白质,为探明其性别决定机制提供蛋白质层面的线索,本研究利用非标记定量蛋白质组学技术,对雌雄同株黄连木上雌花和两性花在两个花芽分化关键物候期中的差异蛋白表达及其相关通路进行了定量分析。结果表明:在5月下旬雄性器官分化的过程中与抗氧化应激、核糖体活动以及光合作用相关的蛋白出现了上调;而在第2年3月上旬雌性器官分化的过程中与抗氧化应激相关的蛋白出现了上调,与光合作用相关的蛋白出现了下调。与第2年3月上旬相比,第1年5月下旬雌花和两性花中与光合作用相关的蛋白均显著上调,与核糖体活动相关的蛋白均显著下调。

The experiment was conducted to identify the differently expressed proteins in flower buds in two key sex differentiation phases in monoecious Pistacia chinensis Bunge, and to provide some clues to depict the sex determination process of P. chinensis . The label-free quantification technique was used in proteomic analysis. The proteins related to oxidative stress resistance were up-regulated while proteins involved in photosynthesis down-regulated during the female primordium differentiation of P. chinensis in early March, while proteins related to oxidative stress resistance, ribosome activity, and photosynthetic function were up-regulated during the male primordium differentiation of P. chinensis in late May. The most up-regulated proteins all involved in the photosynthesis pathway in both kind of flower buds in late May compared to those in early March, and the down-regulated proteins all involved in the ribosome pathway.