柚皮苷和柚皮素对枸橼酸喷托维林经大鼠肝微粒体代谢的影响

Effect of naringin and naringenin on pentoxyverine citrate metabolism in rat liver microsomes in vitro

目的研究柚皮苷和柚皮素对枸橼酸喷托维林经大鼠肝微粒体代谢的影响。方法用高效液相色谱(HPLC)测定底物的量,并根据Lineweaver-Burk方程式计算米氏常数(K m);采用体外温孵法分别评估柚皮苷和柚皮素对枸橼酸喷托维林经大鼠肝微粒体代谢的影响。结果枸橼酸喷托维林与内标分离良好且温孵体系内无其他内源性物质干扰;测得枸橼酸喷托维林体外酶动力学参数:V max为1.74 nmol·(min·mg)^-1,K m为29.00μmol·L^-1;柚皮苷和柚皮素对枸橼酸喷托维林经大鼠肝微粒体的体外代谢产生无明显影响。结论柚皮苷和柚皮素不影响枸橼酸喷托维林在大鼠肝药酶中的代谢。

Objective To investigate the effect of naringin and naringenin on pentoxyverine citrate metabolism in rat liver microsomes in vitro.Methods An HPLC method was used to determine the residual content of pentoxyverine citrate.The Michaelis constant(K·m)for pentoxyverine citrate was calculated according to Lineweaver-Burk equation.The effect of naringin and naringenin on pentoxyverine citrate metabolism was evaluated by rat liver microsome incubation system in vitro.Results The chromatograms showed that pentoxyverine citrate and inter standard were well separated from each other,and both were not disturbed by endogenesis substances.The enzyme kinetic parameters of pentoxyverine citrate in rat liver microsomes,V max and K·m,were 1.74 nmol·(min·mg)^-1 and 29.00μmol·L^-1,respectively.Naringin and naringenin had no significant influence on the metabolism of pentoxyverine citrate by rat liver microsomes.Conclusion Naringin and naringenin did not affect the metabolism of pentoxyverine citrate by rat liver microsomes.