骨细胞MLO-Y4在不同强度静磁场下的生物学效应

Biological Effects of MLO-Y4 Osteocytes in Static Magnetic Field with Different Intensities

目的研究骨样细胞系MLO-Y4在不同强度静磁场下的生物学效应。方法MLO-Y4细胞接受场强为500nT亚磁场、0.2T中强磁场及16T强磁场作用后,CCK8法检测细胞活性,HE染色分析细胞形态变化,实时定量PCR方法检测骨细胞功能相关基因的表达,ELISA或化学试剂盒检测骨细胞可溶性细胞因子的分泌,收集处理后的条件培养基诱导成骨细胞矿化及破骨细胞形成。结果与对照组相比,亚磁场对骨细胞活性和形态无影响,促进了RANKL而抑制了Cx43的基因表达,促进了细胞因子M-CSF的分泌,其条件培养基对成骨细胞矿化无影响,但促进了破骨细胞形成。中强磁场对细胞活性无影响,增加了骨细胞突触数目,其条件培养基对成骨和破骨细胞分化都无明显影响。16T强磁场促进了骨细胞活性及细胞突触数目,抑制了RANKL而促进iNOS和Cx43基因表达,促进ALP和NO而抑制M-CSF分泌,其条件培养基对成骨细胞矿化无影响,但抑制了破骨细胞形成。结论骨细胞MLO-Y4在500nT亚磁场下表现为负向的生物学效应,0.2T中强磁场下无明显生物学行为变化,而在16T强磁场下表现为积极的生物学效应。

Objective To investigate the biological effects of MLO-Y4 osteocytes in static magnetic field(SMF)with different intensities.Methods MLO-Y4 osteocytes were subjected to hypomagnetic field(HyMF)of 500 nT,moderate SMF(MMF)of 0.2 T,and high SMF(HiMF)of 16 T.Cell viability was detected by CCK8.Cell morphology was analyzed by HE staining.Detecting the expression of function marker genes in osteocytes by real-time PCR.The secretion of soluble cytokines in osteocytes were detected by ELISA or chemical kits.The conditional medium after culturing osteocytes in SMF was collected to induce osteoblastic mineralization and osteoclastic formation.Results Compared with the control group,there were no significant effects on cell activity and morphology by HyMF of 500 nT.HyMF promoted the gene expression of RANKLand inhibited the gene expression of Cx43,and increased the secretion of cytokine M-CSF.The conditioned medium after culturing osteocytes in HyMF had no effect on osteoblastic mineralization,but promoted osteoclastic formation.In MMF of 0.2 T,cell activity in osteocytes was not affected,but the number of cell processes was increased.Osteoblastic mineralization and osteoclastic formation was not affected by the conditioned medium after culturing osteocytes in MMF.In HiMF of 16 T,cell viability and the number of cell synapses was promoted.HiMF decreased the gene expression of RANKLand increased the gene expression of iNOS and Cx43,and enhanced the secretion of cytokines ALP and NO,but inhibited the secretion of MCSF.The conditioned medium after culturing osteocytes in HiMF had no effect on osteoblastic mineralization,but inhibited osteoclastic formation.Conclusion MLO-Y4 osteocytes exhibit a negative biological effect under 500 nT HyMF and a positive biological effect under 16 THiMF.There is no significant biological effects under 0.2 T MMF.