毛竹PIN基因家族的鉴定与生物信息学分析

Identification and Bioinformatics Analysis of PIN Gene Family in Moso Bamboo(Phyllostachys edulis)

PIN基因家族是植物特有的一类控制生长素输出的跨膜基因家族。毛竹(Phyllostachys edulis)中PIN基因家族的生物信息学分析一直未见报道。本研究以拟南芥、水稻PIN基因家族为参考序列在毛竹基因组数据库中共鉴定出7条PIN基因,并对其系统进化、保守基序以及在毛竹笋中的差异表达模式进行分析,同时通过实时荧光定量聚合酶链式反应(qRT-PCR)检测PIN基因家族在毛竹生长八个时期和主根、侧根、芽点、腋芽四个组织的相对表达量。结果发现,7条PIN蛋白多属于碱性蛋白,大部分均含有明显的跨膜结构特征。进化结果说明毛竹PIN基因家族与水稻、玉米进化关系较为密切,家族基因进化较保守。表达分析表明,除PhePIN3亚家族外,其他基因都在笋快速生长时期高量表达。PhePIN1-1可能参与了生长素从源到库运输的整个过程,PhePIN1-2主要参与根的生长素调控作用,而PhePIN3亚家族可能不直接参与生长素的运输过程,依靠PIN基因家族成员的生长素从源端运输后可能主要在主根发挥作用。本研究结果证明毛竹PIN基因家族在笋生长的过程中至关重要,为开展毛竹生长素相关的基因家族的研究提供了理论依据。

The PIN gene family is a plant-specific transmembrane gene that controls auxin efflux. Bioinformatic analysis of the PIN gene family in moso bamboo(Phyllostachys edulis) has never been reported. In this study, seven PIN genes were identified in the moso bamboo(Phyllostachys edulis) genome database through using PIN genes of Arabidopsis and rice(Oryza sativa) as query sequences to blast Phyllostachys edulis transcriptome. And their phylogenetic relationship, conserved motifs, and differential expression patterns in bamboo shoots were analyzed. At the same time, the relative expression of PIN gene family in the eight stages of growth and main root, lateral root,bud and axillary bud of moso bamboo was detected by quantitative real-time polymerase chain reaction(qRT-PCR). The results showed that most of the seven PIN gene proteins were composed of basic amino acids, and most of them contained obvious transmembrane structural characteristics. The evolutionary results showed that the PIN gene family of Phyllostachys edulis had a close relationship with rice and maize. Expression analysis indicated that all genes except the PhePIN3 subfamily should be highly expressed during the rapid growth period of bamboo shoots. PhePIN1-1 may be involved in the whole process of auxin transport from source to reservoir. PhePIN1-2 is mainly involved in the regulation of auxin in roots, while the PhePIN3 subfamily may not be directly involved in the transport of auxin. The auxin, which relies on members of the PIN gene family, may play a major role in the main root after transport from the source. The results of this study demonstrated that the PIN gene family of Phyllostachys edulis is essential in the process of shoot growth and provides basic data for the study of gene families related to auxin.