稻瘟菌效应蛋白基因在水稻中过表达对植株表型的影响

Effect of Rice Blast Strain Effector Genes-overexpressing in Rice Plants on Phenotype of Rice Transgenic Lines

病原菌效应蛋白在病原菌-寄主互作过程中发挥双重作用,研究效应蛋白基因在植物中过表达对植株表型及抗性影响将为抗病育种提供重要的参考。本研究对稻瘟病菌效应蛋白转基因水稻各个株系进行PCR鉴定,运用q RT-PCR分析了BAS1、BAS4和MoSDT1在转基因水稻各个株系的根、茎和叶中的表达,同时分析了稻瘟病菌效应蛋白基因过表达水稻植株的表型及根部细胞结构。PCR验证了BAS1转基因水稻的3个株系、BAS4转基因水稻的4个株系及MoSDT1转基因水稻的3个株系均为成功的转基因水稻株系。q RT-PCR分析表明,BAS1、BAS4和MoSDT1在相应的转基因水稻各个株系生长后期在茎组织中的表达量最高。同时分析了转基因水稻各个株系的株高、茎长和宽、叶宽和千粒重等形态指标均与野生型水稻和转空载体的水稻株系的表型基本相似。观察统计转基因水稻根尖1 cm和2 cm处细胞结构发现,转基因水稻根尖细胞的内外皮层数较野生型水稻和转空载体水稻株系的内外皮层细胞数多。本研究的开展将为今后深入研究稻瘟病菌效应蛋白基因在水稻植株中过表达对水稻抗逆性及防御系统响应机制研究提供重要的参考。

Pathogen effectors play the dual role in pathogenicity and inducing defense response during interaction of pathogen and host plants, effect of pathogen effector genes-overexpressing in plants on plants phenotypes and resistance, which contribute to provide important gene resources for disease resistance breeding. In this study,BAS1-, BAS4-, Mo SDT1-overexpressing transgenic lines DNAs were detected by PCR, and phenotypes and cell structure of root of transgenic lines were analyzed, the results showed that the BAS1, BAS4 and MoSDT1 were successfully transferred into rice plants by PCR. The results of qRT-PCR showed that expression level of BAS1,BAS4 and MoSDT1 appeared in highest in stems of corresponding transgenic lines. The phenotypes such as plant height, stem length and width, leaf width and the 1 000-grain weight were analyzed, the results showed that the phenotypes of transgenic lines were similar to wild-type rice plant and GFP-transgenic line. The cell structure of root tip of transgenic rice lines showed that number of outer cortical cells and endothelial cells appeared higher than those of wild-type rice and GFP-transgenic line. Our results provide important references for further studying the resistance mechanism of effector genes-overexperssed transgenic rice plants to abiotic and biotic stress and rice defense response.