A novel fluorescent sensor for cAMP based on E.coli CAP protein

基于大肠杆菌CAP蛋白的检测cAMP的新型荧光探针（英文）

cAMP is an important second messenger that is capable of controlling a wide array of cellular processes, including glycogen, sugar and lipid metabolism. Here we report the design and construction of a novel genetically encoded fluorescent sensor for cAMP. The sensor was realized by fusing E. coli CAP protein with cpYFP, and displayed a one-fold fluorescence change towards c AMP binding. Further characterization assays demonstrated that the sensor had high affinity for cAMP and fast response kinetics.The development of our sensor could be a useful supplement to existing methods for cAMP detection.

cAMP是细胞内一种重要的信号分子,作为第二信使调控包括糖代谢、糖原代谢和脂质代谢在内的一系列生理过程。在本文中我们报道一个新颖的检测细胞内c AMP的基因编码荧光探针,这一探针是由大肠杆菌CAP蛋白和cpYFP荧光蛋白组成的融合蛋白,针对cAMP的结合可以产生一倍的荧光信号响应。进一步的表征实验表明,这一探针对c AMP具有很强的结合能力,并且能够实现快速地荧光响应。这一探针的开发将会为目前已有的检测cAMP的手段提供有益的补充。