摘要
A new fluorescence probe 2,2’-(6-(4-(diethylamino) phenyl)-1,3,5-triazine-2,4-diyl)bis(1 H-pyrazole-1,3-diyl) diacetate(EATPA) based on 1, 3, 5-triazine was designed and synthesized. It exhibited distinct fluorescence quenching in the presence of silver ions that can be used for highly sensitive and selective detection of Ag^+. Fluorescence titration and Job’s plot analysis revealed the formation of [Ag(EATPA)2]^+ entity with high binding constant(1.43×10^4 L/mol) and low detection limit(0.882 μmol/L). Furthermore, live-cell imaging experiments demonstrated that EATPA is potentially applicable for the tracking of Ag^+ in living cells.
A new fluorescence probe 2,2’-（6-（4-（diethylamino） phenyl）-1,3,5-triazine-2,4-diyl）bis（1 H-pyrazole-1,3-diyl） diacetate（EATPA） based on 1, 3, 5-triazine was designed and synthesized. It exhibited distinct fluorescence quenching in the presence of silver ions that can be used for highly sensitive and selective detection of Ag+. Fluorescence titration and Job’s plot analysis revealed the formation of [Ag（EATPA）2]+ entity with high binding constant（1.43×104 L/mol） and low detection limit（0.882 μmol/L）. Furthermore, live-cell imaging experiments demonstrated that EATPA is potentially applicable for the tracking of Ag+ in living cells.
基金
Supported by the National Natural Science Foundation of China(21401144)
the Natural Science Foundation of Hubei Province(2013CFB236)
the Independent Research Discipline Cross-funding of Wuhan University(2042017kf0215)
