人参皂苷Rg1通过改善氧化应激、线粒体损伤及炎症反应对大鼠脊髓缺血再灌注损伤的保护作用研究

Research of the ginsenoside Rg1 protective effect on rat spinal cord ischemia-reperfusion injury by improving oxidative stress, mitochondrial damage and inflammatory response

目的:观察人参皂苷Rg1预处理对于大鼠脊髓缺血再灌注损伤后的干预效果,从氧化应激、线粒体损伤及炎症反应等方面探讨人参皂苷Rg1对抗脊髓缺血再灌注损伤的可能机制。方法:构建大鼠脊髓缺血再灌注损伤模型,随机分为假手术组、缺血组、缺血再灌注组及药物组4组。于损伤后6、12、24、48h分别检测血SOD、MDA含量;并取脊髓组织检测SOD、MDA、COX、IL-6、NF-κB水平;行HE染色。结果:人参皂苷Rg1的干预可以使大鼠血清及脊髓组织SOD活性升高、MDA含量减少,脊髓组织COX活性升高、IL-6及NF-κB水平降低、神经细胞萎缩变形减少。结论:人参皂苷Rg1可以通过抑制线粒体损伤,以及减轻大鼠脊髓缺血再灌注损伤后的氧化应激、炎症反应,达到保护神经细胞的目的。

Objective: To evaluate the effect of ginsenoside Rg1 preconditioning on spinal cord ischemia- reperfusion injury model in rats, and investigate the possible mechanisms of ginsenoside Rg1 against SCI focusing on the oxidative stress, mitochondrial injury and inflammation. Method: The rat SCI model was established, SD rats were randomly divided into 4 groups: sham group, ischemia group, ischemia- reperfusion group and drug group. At different time points after reperfusion, the serum was gathered for SOD and MDA;Rats were sacrificed and the injured spinal cords were immediately removed at every reperfusion time points (6/12/24/48 hours), some samples were isolated for HE staining, the rest were frozen in liquid nitrogen for the evaluation of SOD, MDA, COX, IL-6, NF-κB. Result: Ginsenoside Rg1 intervention can make the SOD activity of rat serum and spinal cord tissue increased, MDA level decreased;the COX activity of spinal cord tissue increased, the IL-6 and NF-κB activity decreased;the atrophy and distortion of nerve cells decreased. Conclusion: Ginsenoside Rg1 can inhibit neuron injury after rat SCI through suppressing the mitochondrial injury, oxidative stress and inflammatory response after spinal cord ischemia- reperfusion injury.