非小细胞肺癌淋巴结Beclin 1表达与淋巴结转移的关系

The relationship between Beclin 1 expression and lymph node metastasis in non-small cell lung cancer

目的探索非小细胞肺癌患者肿瘤组织及周围正常组织Beclin 1水平与淋巴结转移的关系。方法收集我院2011年9月至2016年9月共204例非小细胞肺癌手术患者手术标本,其中男116例、女88例,平均年龄(55.3±11.2)岁。根据肺癌淋巴结转移与否将患者分为无淋巴结转移组(N0组),有肺叶间和叶内淋巴结转移,无纵隔淋巴结转移组(N1组),纵隔淋巴结转移组(N2组)。采用Western blotting法检测标本Beclin 1水平,统计分析患者肿瘤组织及淋巴结标本Beclin 1水平差异。结果 204例肺癌患者中,鳞癌36例、腺癌168例。N0组、N1组、N2组三组患者肿瘤组织中Beclin 1水平逐渐降低,差异有统计学意义(P<0.05)。三组患者肺门及肺内淋巴结Beclin 1(N1 Beclin 1)水平对比,N1组和N2组较N0组明显偏低,差异有统计学意义(P<0.01)。三组患者纵隔淋巴结Beclin 1(N2 beclin 1)水平对比,N2组较N0组及N1组明显偏低,差异有统计学意义(P<0.01)。N1组N1Beclin 1水平低于N2组,差异有统计学意义(P<0.01)。N2组N1 Beclin 1水平略低于N2,但差异无统计学意义(P>0.05)。结论 Beclin 1水平可成为判断肺部肿瘤良恶性的一个参考指标,淋巴结Beclin 1水平可作为协助判断肺癌是否存在淋巴结转移的一个重要参考指标。

Objective To explore the relationship between Beclin 1 level and lymph node metastasis in patients with non-small cell lung cancer. Method A total of 204 surgical specimens of patients with non-small cell lung cancer from September 2011 to September 2016 were collected in our hospital. There were 116 males and 88 females. Beclin 1 levels were detected by Western blotting. There were 116 males and 88 females at average age of 55.3±11.2 years. The patients were divided into three groups including a group N0(no lymph node metastasis), a group N1(intralobar and interlobar lymph node metastases, and no mediastinal lymph node metastasis), and a group N2(mediastinal lymph node metastasis). The differences of Beclin 1 levels in tumor tissues and lymph nodes of patients with N0, N1 and N2 were statistically analyzed. Results Among 204 patients of lung cancer, 36 patients were squamous cell carcinoma and 168 patients were adenocarcinoma. The levels of Beclin 1 in tumor tissues of N0, N1 and N2 groups decreased gradually with a statistical difference(P<0.05). In the three groups, the levels of Beclin 1 in the lung hilum and intrapulmonary lymph nodes(N1 Beclin 1) of N1 and N2 groups were less than that of N0 group with a statistical difference(P<0.01). In the three groups, the level of Beclin 1 in the mediastinal lymph nodes(N2 Beclin 1) of N2 group was less than that of the N0 and N1 groups with a statistical difference(P<0.01). In the N1 group, the level of N1 Beclin 1 was less than that of N2 group(P<0.01). In the N2 group, though the level of N1 Beclin 1 was less than N2 Beclin 1, there was no statistical difference(P>0.05). Conclusion Beclin 1 level can be used as a reference index to judge the benign and malignant lung masses, and lymph node Beclin 1 level can be used as an important reference index to help determine whether there is lymph node metastasis in lung cancer.