虾青素对小鼠蛛网膜下腔出血早期脑损伤的保护机制的研究

Protective mechanism of astaxanthin against early brain injury after subarachnoid hemorrhage in mice

目的研究虾青素对小鼠蛛网膜下腔出血(SAH)早期脑损伤的保护机制。方法 ICR雄性小鼠采用随机数字法随机分为四组:蛛网膜下腔出血组、假手术组、蛛网膜下腔出血+溶剂组(DMSO)、蛛网膜下腔出血+虾青素组。通过建立小鼠视交叉注血方法建立蛛网膜下腔出血模型,于手术后24 h记录神经功能评分,并应用末端脱氧核苷酸转移酶介导的生物素脱氧尿嘧啶核苷酸缺口末端标记法(TUNEL)、western blot法检测脑组织中的NOX2蛋白含量、ELISA法检测TNF-a/IL-1β水平,干湿法检测脑组织中含水量。结果与对照组相比,SAH后小鼠神经功能损伤评分加重,脑水肿加重,脑组织中的NOX2蛋白及脑组织中凋亡阳性细胞数也显著增多,TNF-a/IL-1β值明显升高;应用虾青素干预以后,小鼠神经功能改善,脑组织凋亡阳性细胞明显减少。同时,虾青素能够降低SAH后小鼠脑组织中NOX2蛋白,TNF-a/IL-1β水平值也明显降低。结论虾青素对s AH后早期脑损伤(EBI)具有保护作用,其作用机制可能与其抗氧化性有关。

Objective To study the protective mechanism of astaxanthin against early brain injury( EBI) after subarachnoid hemorrhage( SAH) in mice. Methods Male ICR mice were randomly divided into SAH group,sham operation group,SAH + vehicle( DMSO) group,and SAH + astaxanthin group. A SAH model was established by injecting fresh blood into the prechiasmatic cistern. At 24 hours after surgery,neurological function was scored;the terminal deoxynucleotidyl transferase-mediated d UTP-biotin nick end labeling method and Western blot were used to measure the content of NOX2 protein in brain tissue;enzyme-linked immunosorbent assay was used to determine the level of TNF-a/IL-1β;the water content in brain tissue was evaluated by the wet-dry weight method. Results Compared with the control group,the SAH group had a higher score for neurological impairment,more severe cerebral edema,a significantly higher level of NOX2 protein and a significantly larger number of apoptosis-positive cells in brain tissue,and a significantly higher level of TNF-a/IL-1β. In mice treated with astaxanthin,neurological function was improved,and the apoptosis-positive cells in brain tissue were significantly reduced. At the same time,astaxanthin reduced the level of NOX2 protein in brain tissue and the level of TNF-a/IL-1β after SAH. Conclusions Astaxanthin has a protective effect against EBI after SAH,probably by its antioxidant capacity.