MMP-12基因多态性与动脉粥样硬化性脑梗死的相关性

Relationship between MMP-12 polymorphism and atherosclerotic cerebral infarction

目的探讨辽宁地区汉族人群基质金属蛋白酶12(MMP-12)基因的rs660599多态性与动脉粥样硬化性脑梗死(ACI)的相关性。方法在辽宁地区汉族人群中筛选213例ACI患者为病例组,197例同期健康体检者为正常对照组,采用改良多重连接酶反应技术(IMLDR)检测MMP-12基因rs660599多态性,探讨MMP-12基因rs660599的基因型及等位基因差异。结果病例组与对照组rs660599基因型分布(GG基因型、GA基因型和AA基因型)及等位基因(G和A)频率之间差异无统计学意义(P>0.05),进一步构建显性模型及隐性模型,在显性模型及隐性模型下两组差异均无统计学意义(P>0.05)。结论 MMP-12基因rs660599多态性可能与辽宁地区汉族人群ACI无相关性。

Objective To investigate the relationship between rs660599 polymorphism in matrix metalloproteinase 12(MMP-12) gene and atherosclerotic cerebral infarction(ACI) in Liaoning Han population.Methods A total of 213 ACI patients were screened in Liaoning Han population as case group and 197 healthy people were served as control group at the same time.The improved multiplex ligation detection reaction(IMLDR) was used to detect the rs660599 polymorphism in MMP-12 gene to explore the differences in genotype and allele frequencies of rs660599 in MMP12.Results There were no significant differences in rs660599 genotype distributions(GG genotype,GA genotype and AA genotype) and allele(G and A) frequency between two groups(P>0.05) and also in them of the constructed dominant and recessive models.Conclusion The rs660599 polymorphism of MMP-12 gene may not be associated with ACI in Liaoning Han population.