卵巢癌组织中Numb、TFR1表达量的变化及其临床病理价值研究

The changes of Numb and TFR1 expression in ovarian cancer tissue and their clinical pathological value

目的:探讨卵巢癌组织中Numb、TFR1表达量的变化及其临床病理价值。方法:选择在本院及成都医学院附属医院、绵阳市中心医院接受根治性手术治疗的卵巢癌患者90例,同期接受手术治疗的卵巢巧克力囊肿患者115例,留取两组术中病灶组织标本分别作为卵巢癌组、卵巢巧克力囊肿组。检测卵巢病灶组织中Numb、TFR1基因、卵巢癌相关增殖、侵袭及自噬基因表达量。采用Pearson检验评估卵巢癌组织中Numb、TFR1基因表达量与癌细胞增殖、侵袭、自噬活力的相关关系。结果:卵巢癌组病灶中Numb mRNA的表达量低于卵巢巧克力囊肿组,TFR1 mRNA的表达量高于卵巢巧克力囊肿组;增殖基因C3G、Clusterin、FUNDC1、PIWIL4、TNFAIP8 mRNA的表达量高于卵巢巧克力囊肿组,Let-7i mRNA的表达量低于卵巢巧克力囊肿组;侵袭基因BRMS1、XAV939、OVCA1 mRMA的表达量低于卵巢巧克力囊肿组,CUL4A、MTA1 mRNA的表达量高于卵巢巧克力囊肿组;自噬基因FTY720、Beclin1 mRNA的表达量低于卵巢巧克力囊肿组,NAC-1 mRNA的表达量高于卵巢巧克力囊肿组。相关性分析显示,卵巢癌组织中Numb、TFR1基因表达量与癌细胞的增殖、侵袭、自噬活力直接相关。结论:卵巢癌组织中Numb基因表达量异常降低、TFR1基因表达量异常增高,与癌细胞的恶性生物学行为直接相关。

Objective:To investigate the changes of Numb and TFR1 expression in ovarian cancer tissue and their clinical pathological value.Methods:90 patients with ovarian cancer underwent radical operation in our hospital between August 2015 and April 2018,115 patients with ovarian chocolate cyst underwent surgery during the same period,and intraoperative lesion tissue samples of the two groups were collected and enrolled in ovarian cancer group and ovarian chocolate cyst group respectively.The expressions levels of Numb,TFR1 gene as well as ovarian cancer-related proliferation,invasion and autophagy genes in ovarian lesions were detected.Pearson test was used to evaluate the correlation of Numb and TFR1 gene expression in ovarian cancer tissues with proliferation,invasion and autophagy activity of cancer cells.Results:Numb mRNA expression in lesions of ovarian cancer group was lower than that of ovary chocolate cyst group whereas TFR1 mRNA expression was higher than that of ovary chocolate cyst group;proliferation genes C3 G,Clusterin,FUNDC1,PIWIL4 and TNFAIP8 mRNA expression were higher than those of ovary chocolate cyst group whereas Let-7 i mRNA expression was lower than that of ovary chocolate cyst group;invasion genes BRMS1,XAV939 and OVCA1 mRMA expression were lower than those of ovary chocolate cyst group whereas CUL4 A and MTA1 mRNA expression were higher than those of ovary chocolate cyst group;autophagy genes FTY720 and Beclin1 mRNA expression were lower than those of ovary chocolate cyst group whereas NAC-1 mRNA expression were higher than those of ovary chocolate cyst group.Correlation analysis showed that the Numb and TFR1 gene expression in ovarian cancer tissues were directly correlated with the proliferation,invasion and autophagy activity of cancer cells.Conclusion:The abnormal decrease of Numb gene expression and the abnormal increase of TFR1 gene expression in ovarian cancer tissues are directly correlated with the malignant biological behaviors of cancer cells.