冰片对替莫唑胺抗大鼠脑胶质瘤促进作用的研究

Borneol enhances effect of temozolomide against rat glioma

目的探讨冰片对替莫唑胺抗大鼠脑胶质瘤的促进作用。方法建立Wistar大鼠C6脑胶质瘤模型,随机分为冰片联合替莫唑胺组、单用替莫唑胺组、单用冰片组、空白对照组并分别给药10d。处死各组大鼠,取脑胶质瘤并制作石蜡切片,计算肿瘤最大横截面积,同时采用HE染色观察组织病理学改变,TUNEL法染色观察并计算细胞凋亡率。结果与单用冰片组、单用替莫唑胺组、空白对照组比较,冰片联合替莫唑胺组脑胶质瘤最大横截面积均较小(均P<0.05),细胞凋亡率均较高(均P<0.05);与单用冰片组、空白对照组比较,单用替莫唑胺组脑胶质瘤最大横截面积均较小(均P<0.05),细胞凋亡率均较高(均P<0.05)。空白对照组、单用冰片组脑胶质瘤细胞核呈高密度聚集,冰片联合替莫唑胺组细胞核密度降低,单用替莫唑胺组细胞核密度介于两者之间。空白对照组与单用冰片组均基本看不到凋亡细胞,单用替莫唑胺组可见少量凋亡细胞,冰片联合替莫唑胺组凋亡细胞明显增多。结论冰片对替莫唑胺抗大鼠脑胶质瘤具有促进作用,可能与冰片开放血脑屏障、提高大鼠脑组织替莫唑胺浓度有关。

Objective To investigate the effect of borneol on temozolomide against rat glioma.Methods C6 glioma model was established in 20 adult Wistar rats,the tumor-bearing rats were randomly divided into 4 groups with 5 in each group.The animals were treated with borneol combined with temozolomide(B+T group),temozolomide alone(T group),borneol alone(B group)and normal saline(control group)for 10 d,respectively.Gliomas samples were taken after animals sacrificed,and paraffin sections were made.The maximum cross-sectional area of the tumor was calculated,and histopathological changes were observed by HE staining.TUNEL staining was performed and the apoptosis rate calculated.Results The average cross-sectional area of cerebral glioma in B+T group was significantly lower than that in the other 3 groups(P<0.05).HE staining and TUNEL staining showed that compared to other groups the density of tumor cells decreased significantly,the apoptotic cells increased significantly,and the apoptosis rate was significantly higher in B+T group(P<0.05).Conclusion Borneol can enhance the effect of temozolomide against rat glioma,which may be related to its effect of open blood-brain barrier and the increase of temozolomide concentration in rat brain tissue.