摘要
为狗枣猕猴桃查尔酮异构酶1(AkCHI1)功能研究提供参考,利用生物信息学工具和方法,对狗枣猕猴桃查尔酮异构酶进行结构及功能预测。结果表明:狗枣猕猴桃AkCHI1的cDNA全长为1168bp,编码区(CDS)为861bp,由287个氨基酸构成,具有Chalcone2和Chalcone3的保守序列,是Chalcone3(PLN03174)超家族的一员,为亲水性非稳定蛋白。与其他18种植物的查尔酮异构酶氨基酸序列进行对比表明,狗枣猕猴桃与榴莲的亲缘关系较近,而与葡萄等植物的亲缘关系较远。
In order to provide reference for application of function of Chalcone isomerase 1(AkCHI1)from Actinidia kolomikta,the physicochemical properties,hydrophilic/hydrophobic,transmembrane structure,structural functional domain,secondary structure,tertiary structure,protein modification site and homology of zinc finger protein were analyzed and predicted by using bioinformatics tools and methods.The results showed that the length of AkCHI1 cDNA was 1 168 bp and the coding area was 861 bp coding 287 amino acids.AkCHI1 had conserved sequences of Chalcone2 and Chalcone3,and was a member of Chalcone3 superfamilies(ID:11342810),which was a hydrophilic and unstable protein.The comparison of the amino acid sequences of the chalkeone isomerase between ACHI1 and 18 other plants showed that A.kolomikta was closely related to Durio zibethinus,but far from Vitis vinifera and other plants.
作者
刘丹
李然红
陈鑫
王立凤
LIU Dan;LI Ranhong;CHEN Xin;WANG Lifeng(Mudanjiang Normal University,Mudanjiang,Heilongjiang 157012,China)
出处
《贵州农业科学》
CAS
2019年第9期110-113,共4页
Guizhou Agricultural Sciences
基金
黑龙江省教育厅项目（1354MSYYB007、1354MSYYB006、1354ZD005）
黑龙江省教育厅备案项目（1351MSYYB007）
黑龙江药用植物开发与利用研究（1353PT007）
黑龙江省大学生创新创业训练计划项目（201810233053）
