摘要
Soybean is an important oil crop in China, which plays critical roles in China grain market. Therefore, researches on salttolerant soybean cultivars are of great significance and important for the sustainable development and utilization of the agricultural land resources. In this study, transgenic GmIPK-II(IPK-II) soybean seedlings from three single lines and 10 GmHAL-I(HAL-I) transgenic lines were provided by Jilin Academy of Agricultural Sciences. Wild type seedlings of soybean cultivars Dongnong 50(DN50) and Huachun 6(HC6) were the controls. Transgenic and wild-type seedlings were evaluated under different salt concentrations. Moreover, three kinds of culture methods and two kinds of salt application methods including "irrigated from top" and "permeated from bottom" were discussed. Through observing the changes of phenotype from transgenic and control seedlings under different salt concentrations, the salt tolerance screening evaluation system with high efficiency was initially confirmed, which conditions were as the followings: vermiculite and turfy soil were mixed as 1 : 1. Concentration of NaCl was 150 mmol · L-1. Time of the treatments was 6 days. Finally, 23 transgenic lines of GmIPK-II and GmHAL-I with higher salt-tolerance were obtained, which laid a foundation for researches.
Soybean is an important oil crop in China, which plays critical roles in China grain market. Therefore, researches on salttolerant soybean cultivars are of great significance and important for the sustainable development and utilization of the agricultural land resources. In this study, transgenic GmIPK-II（IPK-II） soybean seedlings from three single lines and 10 GmHAL-I（HAL-I） transgenic lines were provided by Jilin Academy of Agricultural Sciences. Wild type seedlings of soybean cultivars Dongnong 50（DN50） and Huachun 6（HC6） were the controls. Transgenic and wild-type seedlings were evaluated under different salt concentrations. Moreover, three kinds of culture methods and two kinds of salt application methods including "irrigated from top" and "permeated from bottom" were discussed. Through observing the changes of phenotype from transgenic and control seedlings under different salt concentrations, the salt tolerance screening evaluation system with high efficiency was initially confirmed, which conditions were as the followings: vermiculite and turfy soil were mixed as 1 : 1. Concentration of NaCl was 150 mmol · L-1. Time of the treatments was 6 days. Finally, 23 transgenic lines of GmIPK-II and GmHAL-I with higher salt-tolerance were obtained, which laid a foundation for researches.
基金
Supported by Heilongjiang Postdoctoral Fund(LBH-Z12047)
Beeding of New Soybean Varieties with Antireversing Genes(2016ZX08004-002)
Heilongjiang Education Department Project(10531012)
