不同剂量原花青素对两岁伊犁马抗氧化的影响

Effects of Different Doses of Procyanidins on Antioxidant Activity of 2-Year-Old Yili Horses

【目的】研究补饲不同剂量原花青素对伊犁马1000m速度赛前后血清抗氧化指标的影响。【方法】选择年龄2岁,1000m速度赛成绩接近的伊犁马公马16匹,随机分为四组。分别以四个剂量组原花青素进行为期28d的补饲,在第28d进行1000m速度赛并进行采样,采样的时刻为赛前30 min、赛后即刻、赛后30min、赛后90min、赛后24h。采集静脉血对相关指标进行测定,对测定结果进行单因素方差分析。【结果】静息状态:SOD活性实验组时显著高于对照组(P<0.05);GSH-PX活性添加60 mg/kg BW和40mg/kg BW剂量组显著高于对照组(P<0.05);运动后30min:SOD、GSH-PX活性添加60 mg/kg BW剂量组时显著高于对照组(P<0.05);MDA含量添加60 mg/kg BW剂量组显著低于对照组(P<0.05)。运动后90min:SOD活性添加60 mg/kg BW剂量组时显著高于对照组(P<0.05);GSH-PX活性添加60 mg/kg BW剂量组极显著高40 mg/kg BW剂量组、20mg/kg BW剂量组和对照组(P<0.01);运动后24h:SOD、GSH-PX活性添加60 mg/kg BW剂量组时显著高于对照组(P<0.05)。【结论】运动马补饲原花青素的量控制在60mg/kg BW可有效提高运动型伊犁马抗氧化能力。

【Objective】 To study the effects of different doses of proanthocyanidins on serum antioxidant indexes before and after 1,000 m speed race in Yili horses.【Method】 Sixteen Yili horse horses, aged 2 years and close to 1,000 m speed, were randomly divided into four groups. Four dose groups of proanthocyanidins were supplemented for 28 days, and 1,000 meters speed race was carried out on the 28 th day. The sampling time was 30 minutes before the race, 30 minutes after the race, 90 minutes after the race and 24 hours after the race. Venous blood was collected to determine the related indexes, and the results were analyzed by single factor variance analysis.【Result】 In resting state: the activity of SOD in the experimental group was significantly higher than that in the control group(P < 0.05). GSH-PX were significantly higher in the 60 mg/kg BW and 40 mg/kg BW than control group(P <0.05). 30 min after the game: SOD and GSH-PX were significantly higher in the 60 mg/kg BW and 40 mg/kg BW than the control group(P<0.05). MDA content was significantly lower in the 60 mg/kg BW than control group(P <0.05). 90 min after the game: SOD were significantly higher in the 60 mg/kg BW than the control group(P <0.05). GSH-PX were significantly higher in the 60 mg/kg BW than 40 mg/kg BW and the control group with 20 mg/kg BW(P <0.05). 24 hours after the game: SOD and GSH-PX were significantly higher in the 60 mg/kg BW than the control group(P <0.05).【Conclusion】 The amount of procyanidins supplemented to exercise horses was controlled at 60 mg/kg BW can effectively promote the antioxidant capacity of the Yili horses.