CVB对OL细胞和OL/BDV细胞I型IFN信号转导途径的影响

Effect on Type I IFN Signal Transduction Path in OL and OL/BDV Cells Infected with CVB

探讨柯萨奇病毒(coxsackievirus, CVB)对人类少突胶质细胞(oligodendrocyte, OL)和博尔纳病病毒(Borna disease virus, BDV)持续感染的OL细胞(OL/BDV)中I型干扰素(interferon, IFN)、microRNA-155(miR-155)表达以及干扰素调节因子(interferon regulatory factor, IRF)7细胞定位的影响。CVB感染OL细胞和OL/BDV细胞0、2、4、6、12和24 h,qPCR检测I型IFN mRNA和miR-155表达水平。OL细胞和OL/BDV细胞分别转染IRF7-EGFP质粒,CVB感染4 h,观察IRF7细胞定位情况。CVB感染OL细胞2、4、12和24 h,IFN-αmRNA和miR-155表达水平显著增加,IFN-βmRNA表达水平在4、12和24 h显著增加;CVB感染OL/BDV细胞IFN-α和IFN-βmRNA表达水平除0 h均显著增加,但miR-155表达仅在12和24 h显著增加。CVB感染OL细胞和OL/BDV细胞4 h,IRF7绿色荧光蛋白由细胞浆转移至细胞核内。综上所述,CVB可诱导OL细胞和OL/BDV细胞I型IFN、miR-155的表达,促进IRF7入核,从而激活I型IFN信号转导途径。

Effects of coxsackievirus(CVB) on the expression of type I interferon(IFN) and microRNA-155(miR-155) as well as the localization of interferon regulatory factor(IRF) 7 in human oligodendrocyte(OL) and Borna disease virus(BDV) infected OL cells(OL/BDV) were investigated. After the CVB infection, the expression of type I IFN mRNA and miR-155 in OL cells and OL/BDV cells were detected by qPCR on 0, 2, 4, 6, 12 and 24 h and observed the localization of IRF7 cells. OL cells and OL/BDV cells were respectively transfected to IRF7-EGFP plasmid and infected with CVB for 4 h and observed the cell localization. The expression of IFN-α mRNA and miR-155 significantly increased at 2, 4, 12 and 24 h, and IFN-β mRNA significantly increased at 4, 12 and 24 h in OL cells. The expression of IFN-α and IFN-β mRNA in OL/BDV cells infected with CVB all increased significantly at most time points detected except 0 h, however, miR-155 increased only at 12 and 24 h. In OL cells and OL/BDV cells infected with CVB for 4 h, IRF7 green fluorescent protein was translocated from cytoplasm into nucleus. In summary of all above, CVB could induce the expression of type I IFN and miR-155 and promote the entrance of IRF7 into nucleus, thus activated the type I IFN signal transduction pathway.