解脂亚罗酵母菌体外降甘油三酯降胆固醇效果研究

Effect of Yarrowia lipolytica on Triglyceride and Cholesterol-Reduction in vitro

筛选出高效降三酰甘油降胆固醇菌株,为新型药物、食品的制备提供优良菌株。从样品中筛选分离出酵母菌株,并测定菌株降三酰甘油能力,采用磷硫铁比色法测定菌株降胆固醇能力,通过耐酸、耐胆盐及毒性实验研究菌株各项性能,通过生理生化及26S rDNA法对菌株进行鉴定。结果显示,试验筛选出1株高效降脂降胆固醇酵母菌EAM-ZT003T,降三酰甘油能力高达80.2%,菌株具有很好的耐酸、耐胆盐能力,为期42 d的小鼠毒性试验发现,小鼠未出现异常体征,未发现异常死亡,且解剖观察各器官一切正常,菌株经26S rDNA鉴定为解脂亚罗酵母菌(Yarrowia lipolytica)。

Triglyceride and cholesterol-lowering excellent strains was screened in order to prepare new drugs and food. Isolation and purification of yeast strains from samples and test for its triglyceride degradation ability of the target strains, and the cholesterol lowering ability was determined adopted phosphorus-sulfur-iron colorimetry. The screened strains were further investigated for their tolerance to acid and bile salt, and toxicity test and other performances. Physiological, biochemical tests, and 26 S rDNA method were used to identify the strains. The results showed that the experiment had screened a triglyceride and cholesterol degradable strain EAM-ZT003, particularly the triglyceride degradation ability was as high as 80.2%. And the results showed the strain had fine tolerance to acid and bile salt. And it was found that in 42 d of toxicity test in mice and revealed no abnormal pathological sign and exceptional death, and all the dissected organs of mice were observed to be normal. The strain EAM-ZT003 T was identified as Yarrowia lipolytica adopting 26 S rDNA.